o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
4. ECOTOXICITY ID: 95-53-4
DATE: 07.01.2005
120
Flag : Critical study for SIDS endpoint
10.11.2004 (115) (111) (113)
Type : aquatic
Species : Tetrahymena pyriformis (Protozoa)
Exposure period : 40 hour(s)
Unit : mg/l
EC50 : ca. 155
Analytical monitoring : no
Method : other: Population growth impairment assay
Year : 1999
GLP : no data
Test substance : other TS: o-Toluidine, purity > 95 %
Method : Test was performed according to the method described by Schultz TW
(1997). TETRATOX: Tetrahymena pyriformis population growth impairment
endpoint. A surrogate for fish lethality. Toxicol. Methods 7, 289-309.
- The test was conducted in foam-stoppered 250 ml Erlenmeyer flasks
containing 50 ml of sterile, semidefined proteose-peptone-based medium.
- Test culture was inoculated with log-growth-phase culture. Initial density
varied from 1000 to 5000 cells/ml.
- The temperature was maintained at 27+/-1 °C. Medium was buffered to
pH 7.4 prior to sterilization.
- The assay was conducted as a 40 h static test.
Result : Result was given as log 1/IGC50 = -0.16, IGC50 in mM. IGC50 = 50 %
growth inhibition concentration
Test condition : 200 substances were tested to derive QSAR.
Test was performed using the freshwater ciliate Tetrahymena pyriformis
(Strain GL-C)
The test conditions were as follows:
- non-neutralised, allow for 8-9 cell cycles in control cultures.
- The pH of the test media was 7.3 and was not controlled during the test.
Prior to testing in duplicate for three replicates, the compound was tested in
a range finding test. Test replicates consisted of 6 to 8 concentrations with
duplicate flasks of each concentration.
- The endpoint population density was measured spectrophotometrically at
540 nm.
Test substance : The stock solution was presumably dissolved in < 7.5 ml/l DMSO
(dimethlysulfoxide).
Reliability : (2) valid with restrictions
Basic data given
Flag : Critical study for SIDS endpoint
11.02.2004 (34) (116) (117)
Type : aquatic
Species : Tetrahymena pyriformis (Protozoa)
Exposure period : 24 hour(s)
Unit : mg/l
EC50 : = 520
Analytical monitoring : no
Method : other: cell multiplication inhibition test
Year : 1985
GLP : no data
Test substance : other TS: o-Toluidine, analytical grade
Method : - 2 different counting methods were used. One used a microscope with a
specially prepared glass slide which contained a depression (20x50 mm)
that could hold 20 cells. A quantity of cultured medium was dropped onto
the glass slide and an adequate amount of 3.7 % formaline and 0.7
% CaCl2 was added to kill the cells. The count was repeated 3 times and
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