o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
4. ECOTOXICITY ID: 95-53-4
DATE: 07.01.2005
122
nominal TS concentration
Test condition : - Stock and preliminary cultures of protozoa are fed with viable bacteria
(Escherichia coli).
- Temperature = 25 °C; initial pH = 6.9 (adjusted)
- The number of protozoa is determined by means of a cell counter (Coulter
counter) after addition of a suitable electrolyte (10 % of a 1.1 % NaNO3
solution in double-distilled water filtered through a membrane filter (pore
size = 0.2 µm)
Reliability : (2) valid with restrictions
Study meets generally accepted scientific principles
Flag : Critical study for SIDS endpoint
30.01.2004 (121) (122) (113) (120) (114)
Type : aquatic
Species : Chilomonas paramaecium (Protozoa)
Exposure period : 48 hour(s)
Unit : mg/l
EC5 : = 237
Analytical monitoring : no
Method : other: cell multiplcation inhibition test according to Bringmann (1978)
Year : 1980
GLP : no
Test substance : other TS: o-Toluidine, purity is not specified
Method : Bringmann G (1978). Bestimmung der biologischen Schadwirkung
wassergefährdender Stoffe gegen Protozoen. I.Bakterienfressende
Flagellaten (Modellorganismus: Entosiphon Sulcatum Stein). Z. Wasser u.
Abwasser-Forsch.11, 210-215.
Result : Reported TT (Toxicity Threshold) is comparable to EC5; value refers to
nominal TS concentration
Test condition : - Temperature = 20 °C; pH = 6.9 (adjusted)
- The number of protozoa is determined by means of a cell counter (Coulter
Counter) after addition of a suitable electrolyte (10 % of a 1.1 % NaNO3
solution in double-distilled water filtered through a membrane filter (pore
size = 0.2 µm))
Reliability : (2) valid with restrictions
Study meets generally accepted scientific principles
Flag : Critical study for SIDS endpoint
29.01.2004 (123) (120)
Type : aquatic
Species : other protozoa: Spirostomum ambiguum
Exposure period : 24 hour(s)
Unit : mg/l
LC50 : 5411
Analytical monitoring : no
Method : other: Spirotox test
Year : 1999
GLP : no
Test substance : other TS: o-Toluidine, purity is not specified
Result : Results were given in mmol/l after 24 and 48 h.
For the deformation differences:
24 h and 48 h-EC50= 34.6+/-6.82 mmol/l = 3707.4+/-731 mg/l
For lethality:
24 h and 48 h-EC50= 50.5+/-24.9 mmol/l = 5411+/-2668 mg/l
Test condition : - Test organism: Spirostomum ambiguum, one of the biggest protozoans
(2-3 mm long)
- Diluent: Tyrod solution: 125 mg NaCl, 3.1 mg KCl, 3.1 mg CaCl2, 1.55 mg
MgCl2, 15.6 mg NaHCO3 and 0.78 mg NaH2PO4 per liter of deionised
water. Total hardness = 2.8 mg CaCO3/l and pH = 7.4 +/- 0.2.
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