o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

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OECD SIDS o-TOLUIDINE 5. TOXICITY ID: 95-53-4 DATE: 07.01.2005 218 HU/ara-C at 1.70 mM and 4.24 mM Test condition : in presence (to increase the sensitivity) or in absence of the DNA-repair inhibitors - hydroxyurea and cytosine arabinoside (HU/araC) toxicity is measured as cell viability parallel to the comet assay no data on positive control Reliability : (2) valid with restrictions special study 15.07.2004 (297) Type : other: DNA damage System of testing : human breast milk cells Test concentration : 0.85 mM Cycotoxic concentr. : > 0.85 mM Metabolic activation : without Result : positive Method : other: alkaline single-cell gel electrophoresis (comet) assay according to Singh et al.,1988, Exp. Cell Res. 175, 184-191 Year : 2000 GLP : no data Test substance : other TS: o-toluidine, no data on purity Result : Comet tail length were significantly increased in the presence of the DNArepair inhibitors - hydroxyurea and cytosine arabinoside (HU/araC) and in absence of cytotoxicity (83% of cells viable) Reliability : (4) not assignable special study 15.07.2004 (298) (299) Type : other: DNA damage System of testing : human and rat epithel cells of the urinary bladder Test concentration : 8, 16 and 32 mM Cycotoxic concentr. : > 80 mM Metabolic activation : Result : positive Method : other: alkaline single-cell gel electrophoresis (comet) assay according to Singh et al.,1988, Exp. Cell Res. 175, 184-191 Year : 2002 GLP : no data Test substance : other TS: o-toluidine, purity as reagent grade Result : Significant dose-dependent increases of DNA fragmentation were obtained in cells from rats at 8, 16 and 32 mM and in cells from humans with concentrations from 16 and 32 mM. Test condition : Primary human and rat epithel cells of urinary bladder were incubated with test substance over a time period of 20 hours. DNA damge was quantified by the increase of tail moment, that was defined as the product of the tail length and the amount of DNA in the tail (Olive et al., 1990). Statistical analysis was performedby the use of analysis of variance followed by Dunnet's test. solvent: DMSO Reliability : (2) valid with restrictions meets scientific principles 15.07.2004 (300) Type : other: DNA single-strand breaks UNEP PUBLICATIONS
OECD SIDS o-TOLUIDINE 5. TOXICITY ID: 95-53-4 DATE: 07.01.2005 System of testing : rat hepatocytes Test concentration : 0, 0.313, 0.625, 1.25, 2.5, 5, 10 mM in DMSO Cycotoxic concentr. : >10 mM in DMSO Metabolic activation : without Result : negative Method : other: alkaline elution technique as described Stout and Becker, 1982, Analytical Biochemistry 127, 302-307,see freetext ME Year : 1994 GLP : no data Test substance : other TS: o-toluidine, no data on purity Method : Hepatocytes fron 6-7 weeks old female SD rats Treatment time in the presence of TS: 3 hours Solvent: DMSO Controls: negative controls: solvent positive control: Dimethylnitrosamine Evaluation as positive if a three-fold increase in elution rate when compared to the concurrent negative control Result : The calculated elution slopes in the cells treated with o-toluidine for 3h were less then 3 times the control slope at all concentrations: o-toluidine did not induce DNA single-strand breaks in rat hepatocytes under test conditions. the positive control was functional. Reliability : (2) valid with restrictions no data on purity and no information on GLP 15.07.2004 (215) (216) Type : other: DNA single-strand breaks System of testing : Chinese hamster ovary (CHO) cells Test concentration : 0.02 and 0.05 M (approximately 2140 and 13200 µg/ml) Cycotoxic concentr. : >0.05 M Metabolic activation : with and without Result : positive Method : other: alkaline sucrose sedimentation Year : 1985 GLP : no data Test substance : other TS: o-toluidine, no data on purity Test condition : METABOLIC ACTIVATION: S9 mix liver homogenates from Aroclor 1254 induced rats Positive controls - diethylnitrosamine and methylmethanesulfonate Negative controls - untreated CHO cells TEST CRITERION A positive result was indicated by a dose-related increase in the number of ssb, the minimum significant amount of ssb being 5 ssb/10E9 dalton DNA. Reliability : (4) not assignable Method evaluation
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OECD SIDS o-TOLUIDINE FOREWORD INTR
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OECD SIDS o-TOLUIDINE 11. Comments:
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OECD SIDS o-TOLUIDINE 6 REFERENCES
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OECD SIDS o-TOLUIDINE Bringmann G,
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OECD SIDS o-TOLUIDINE Hecht SS, El-
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OECD SIDS o-TOLUIDINE phase extract
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OECD SIDS o-TOLUIDINE Stark M (1892
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OECD SIDS o-TOLUIDINE I U C L I D D
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OECD SIDS o-TOLUIDINE 1. GENERAL IN
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o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

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