o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
5. TOXICITY ID: 95-53-4
DATE: 07.01.2005
CONTROLS:
positive controls:
without S9-mix:
TA1537 - 9-aminoacridine
TA98 and TA1538 - 2-Nitrofluorene
TA100 and TA1535 - N-Methyl-N'-nitro-N-nitrososguanidine
with S9-mix
all strains - 2-Aminoanthracene
The positive controls increased mutant counts to well over those of the
negative controls, and thus demonstrated the system's sensitivity and the
activity of the S9 mix.
negative controls: solvent DMSO
CRITERIA FOR POSITIVE RESPONSE:
dose-related increase in revertants exceeding control values
by at least two-fold in at least two successive concentrations of the test
chemical.
Reliability : (2) valid with restrictions
Toxicity was not reached, no data on purity of TS
Flag : Critical study for SIDS endpoint
21.05.2004 (170)
Type : Ames test
System of testing : Salmonella typhimurium TA98, TA100, TA1535, TA1537
Test concentration : (1) 0, 33, 100, 333, 1000, 3333 µg/plate in DMSO
(2) 0, 100, 333, 1000, 3333, 6666 µg/plate in DMSO
Cycotoxic concentr. : cytotoxicity was determined in preliminary experiments (no further
information)
Metabolic activation : with and without
Result :
Method : other: preicubation protocol according to Haworth, 1983 Environ.
Mutagen.5 [Suppl 1], 3-142, see freetext ME
Year : 1992
GLP : no data
Test substance : other TS: o-toluidine hydrochloride, purity: 99 %
Method : -----Preincubation procedure:
incubation time with TS: 20 min
TS was testes with and without S9-mix
-----Metabolic activation systems:
S9-mix was prepared from Aroclor 1254-induced male Sprague -Dawley
rats(RLI) and males Syrian Hamster(HLI) in 10 % and 30 % concentrations.
-------Controls:
Positive controls were used, but the name of the substances were not
mentioned.
negative controls: solvent: DMSO
-----Evaluation of the results
A chemical was judged mutagenic or weakly mutagenic if it produced a
reproducible dose-related response over the solvent control.
o-toluidine hydrochloride was tested in 2 different laboratories:
(1) Salmonella Typhimurium TA 98, TA100, TA 1535, TA 1537 without
metabolic activation and in the presence of 10 % RLI and 10 % HLI,
positive and negative controls
(2) Salmonella typhimurium TA 98, TA100 without metabolic activation and
in the presence of 30 % RLI and 30 % HLI, positive and negative controls
Result : (1) o-toluidine hydrochloride was tested negative in all strains with and
sithout RLI and HLI except the questionable result in TA100 in the
presence of HLI
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