o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
OECD SIDS o-<strong>TOLUIDINE</strong><br />
3. ENVIRONMENTAL FATE AND PATHWAYS ID: <strong>95</strong>-<strong>53</strong>-4<br />
DATE: 07.01.2005<br />
78<br />
- Derivatisation of free amines with trifluoroacetic anhydride<br />
- GC (Perkin Elmer F11, stainless steel capillary columns, several<br />
stationary phases)<br />
Result : o-Toluidine occurs in tocacco leaves<br />
Reliability : (2) valid with restrictions<br />
Basic data given<br />
Flag : Critical study for SIDS endpoint<br />
24.02.2004 (44)<br />
Type of measurement : other: natural occurrence<br />
Media : food<br />
Concentration :<br />
Method : GC/MS<br />
Method : - Commercial blend of black tea was extracted with supercritical CO2 at<br />
100-300 bars<br />
- Separation from CO2 at 50-70 bars to obtain aroma concentrate<br />
- Steam distillation of aroma concentrate<br />
- Fractionation in basic and neutral fraction<br />
- Direct injection of fraction into GC<br />
- GC (Carlo Erba GI Model 450, glass capillary column coated with<br />
polypropylene glycol)<br />
- GC coupled to MS<br />
Result : o-Toluidine occurs in the aroma of black teas<br />
Reliability : (2) valid with restrictions<br />
Basic data given<br />
Flag : Critical study for SIDS endpoint<br />
24.02.2004 (45)<br />
Type of measurement : concentration at contaminated site<br />
Media : ground water<br />
Concentration :<br />
Method : reverse phase HPLC<br />
Method : In the environment o-toluidine is formed by reduction of o-nitrotoluene, e.g.<br />
at former munitions sites. Study was performed to elucidate the role of the<br />
manganese peroxidase (MnP) from white-rot fungi (species not reported).<br />
An abiotic system consisting of Mn(III) in oxalate buffer under aerobic<br />
conditions (Mn(II)/oxalate/O2) was used to transform 2-amino-4,6dinitrotoluene<br />
and its derivatives (including o-toluidine).<br />
Remark : Situation at former munitions sites is briefly described<br />
Result : o-Toluidine was degraded much faster than its precursor, o-nitrotoluene<br />
Test condition : - 50 µM (5.35 mg/l) of the test substance were incubated for 96 h at 20 °C<br />
in a system containing oxalate and Mn(III), under aerobic conditions<br />
- The reaction mixture was sterilized<br />
- pH rose from 4.5 to 7.5-8.0 over the time of the experiment<br />
- Quantitative determination of nitroaromatic compounds by reversed<br />
phase HPLC<br />
Reliability : (2) valid with restrictions<br />
Basic data given<br />
Flag : Critical study for SIDS endpoint<br />
20.06.2004 (46)<br />
Type of measurement : other: concentration in fuel<br />
Media : other: coal oil<br />
Concentration :<br />
Method : GC/MS<br />
Method : - Acidic extraction<br />
- Gel chromatography for purification<br />
- Derivatisation with trifluoroacetic anhydride<br />
<strong>UNEP</strong> PUBLICATIONS
Change language