o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
3. ENVIRONMENTAL FATE AND PATHWAYS ID: 95-53-4
DATE: 07.01.2005
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about 1 g of the soil was transferred to liquid medium with 0.4 g otoluidine/l.
When degradation in this culture was complete, it was
transferred and after several transfers a pure bacterial culture was
obtained.
- Growth conditions: The strain was grown at 30 °C in mineral salts medium
containing 0.4 g/l o-toluidine as sole carbon source. Stock cultures were
maintained on the medium supplemented with 1.5 % agar; mineral salts
medium.
-Resting cells
For resting-cell experiments, o-toluidine induced cells were prepared by
adding 0.1 g o-toluidine to 1 l of medium containing 2g/l glucose during the
early exponential growth phase. The cells were harvested, washed with
buffer, and resuspended in the buffer containing o-toluidine as substrate (1
g/l). Thev suspension was incubated at 35°C.
Isolation and identification of compounds was performed via analytical TLC
and GC/MS.
Reliability : (2) valid with restrictions
Test procedure in accordance with generally accepted
scientific standards and described in sufficient detail
15.06.2004 (76)
Type : aerobic
Inoculum : other: water of the river Elbe, Germany
Contact time :
Degradation : 50 (±) % after 48 minute(s)
Result :
Deg. product :
Method : other: Biodegradation in River Water. Simulation experiment (Testfilter)
Year : 2001
GLP : no
Test substance : other TS: Group of substances including o-toluidine (Purity >97 %)
Remark : Compared to common batch tests the advantages of testfilter experiments
are their short duration and the use of a population of microorganisms
adapted to the river or wastewater. These simulate the biological
degradation process which occurs in the river bed sediments under aerobic
conditions.
Result : A half life time (t1/2) of 0.8 h (mean of 17 determinations) and a mean
degradation rate of 0.90 h-1 were determined for o-toluidine.
Test condition : - Experiments were carried out in circulating water through a glass column
filled with pumice under darkness conditions and at a temperature of 20 °C.
- Stable aerobic conditions were proven by measuring dissolved oxygen
concentrations in the column discharge.
- The pumping rate was 3 ml/min.
- Unfiltered water from the river Elbe, Germany, was circulated for a
minimum of 1 month, and replaced by fresh river water every 6 to 8 days.
- Water was spiked with single substances or mixtures in concentrations of
3 to 24 µg/l per substance.
- The concentration of o-toluidine was followed by HPLC/DAD (DAD =
diode array detector)
- Limit of detection = 0.28 µg/l in Boernick, Eppinger, Grischek, and Worch
(2001); limit of detection = 0.028 µg/l in Boernick, Grischek, and Worch
(2001)
Reliability : (2) valid with restrictions
Study meets generally accepted scientific principles
20.02.2004 (77) (54)
Type : aerobic
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