o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

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OECD SIDS o-TOLUIDINE 5. TOXICITY ID: 95-53-4 DATE: 07.01.2005 214 o-toluidine was tested positive in the presence of S9-mix although no dose response relationship was noted. The positive controls were reported to be fucntional. Test condition : This study is part of a International Collaborative Program, published as: Evaluation of Short-Term Tests for Cancerogens Progress in Mutation Research Vol 1. Edited by Frederick J. Serres and John Ashby, 1981 CHEMICALS: 42 coded chemicals were investigated in short-term tests for cancerogenicity in different laboratories It should be suggested that only 100%-pure chemicals should be used when evaluating a test system. ---In the present work the compound with code no 41 is declared as otoluidine hydrochloride, whereas in an the overall summary of this program code no. 41 is declared as o-toluidine. METABOLIC ACTIVATION: S9 mix - liver homogenates from with phenobarbitone induced male Wistar rats Controls: negative control: solvent (DMSO) positive controls: in the presence of S9-mix: cyclophosphamide in the absence of S9-mix: 4-nitroquinoline-1-oxide Incubation time: 2.5 hours Stastistical analysis: student t'Test Reliability : (4) not assignable Method-Evaluation, no data on test concentration, no data on cytotoxicity, unclear use: o-toluidine or o-toluidine hydrochloride 15.07.2004 (152) (290) Type : Unscheduled DNA synthesis System of testing : primary rat hepatocytes Test concentration : 0.02 - 0.000001 M in DMSO Cycotoxic concentr. : no data Metabolic activation : without Result : positive Method : other: according Althaus et al., 1982, Cancer Res. 42, 3010 Year : 1985 GLP : no data Test substance : other TS: o-toluidine, no data on purity Result : significant increase; 176% of control induced UDS at the optimal concentration Test condition : Incubation time: 18 hours Nuclear isolation followed by scintillation counting of extracted DNA Metabolic activation: intrinsic Controls: UV Doses tested 0.02 - 0.00002 M for dose-response curve, max effective dose for further testing 0.002 M Definition of positive results: Means at maximum effective dose compared by a 1-sided Student`s t-test or Lohrding`s test. A sample mean which was greater than a control mean with > 95% confidence was considered positive UNEP PUBLICATIONS
OECD SIDS o-TOLUIDINE 5. TOXICITY ID: 95-53-4 DATE: 07.01.2005 Reliability : (2) valid with restrictions test procedure evaluation 15.07.2004 (291) (280) Type : Unscheduled DNA synthesis System of testing : HeLa S3 cells Test concentration : 0.01, 0.05, 0.1, 0.5, 1 µl/ml in DMSO Cycotoxic concentr. : preliminary cytotoxicity test, no data Metabolic activation : with and without Result : positive Method : other: according Martin et al., 1977 Year : 1985 GLP : no data Test substance : other TS: o-toluidine, no data on purity Result : o-Toluidine induced reproducible statistically significant increases in UDS in the presence of S9. Test condition : METABOLIC ACTIVATION: S9 mix liver homogenates from Aroclor 1254 induced rats CONTROLS: positive - MNNG, B(a)P negative - concurrent solvent controls Definition of positive results: Statistically significant (F-test) dose-related increase in 3H-TdR incorporation Reliability : (2) valid with restrictions test procedure evaluation 15.07.2004 (292) (280) Type : Unscheduled DNA synthesis System of testing : rat hepatocytes Test concentration : 0.05, 0.1, 0.53, 1.07, 5.35, 10.7, 53.5, 107 µg/ml DMSO Cycotoxic concentr. : 107 µg/ml DMSO Metabolic activation : Result : negative Method : other: autoradiographic assay for UDS Year : 1985 GLP : no data Test substance : other TS: o-toluidine, no data on purity Method : Incubation time: 20 hours Positive control substances: N-methy-N'-nitro-N-nitrosoguanidine (MNNG), 2-acetylaminofluorene (AAF) Quantification as described by Probst et al(1981),Environ. Mutagen 3, 11 Criteria for positive response: 2 successive compound concentrations produced nuclear grain counts which exceed those of the control by 3 standard deviations of the control value Result : the positive controls were functional Reliability : (2) valid with restrictions test procedure evaluation 15.07.2004 (293) (280) Type : Unscheduled DNA synthesis System of testing : primary rat- and hamster hepatocytes in vitro UNEP PUBLICATIONS 215
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OECD SIDS o-TOLUIDINE FOREWORD INTR
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OECD SIDS o-TOLUIDINE 11. Comments:
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OECD SIDS o-TOLUIDINE and transitio
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OECD SIDS o-TOLUIDINE countries), e
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OECD SIDS o-TOLUIDINE 1.3 Physico-C
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OECD SIDS o-TOLUIDINE With adapted
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OECD SIDS o-TOLUIDINE 3.1.2 Acute T
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OECD SIDS o-TOLUIDINE 3.1.4 Sensiti
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OECD SIDS o-TOLUIDINE Inhalation Th
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OECD SIDS o-TOLUIDINE Conclusion Th
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OECD SIDS o-TOLUIDINE 4.2 Terrestri
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OECD SIDS o-TOLUIDINE 6 REFERENCES
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OECD SIDS o-TOLUIDINE Bringmann G,
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OECD SIDS o-TOLUIDINE Hecht SS, El-
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OECD SIDS o-TOLUIDINE phase extract
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OECD SIDS o-TOLUIDINE Stark M (1892
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OECD SIDS o-TOLUIDINE I U C L I D D
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OECD SIDS o-TOLUIDINE 1. GENERAL IN
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OECD SIDS o-TOLUIDINE 5. TOXICITY I
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o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

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