o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
4. ECOTOXICITY ID: 95-53-4
DATE: 07.01.2005
Unit : mg/l
LC50 : 82.5
Limit test :
Analytical monitoring : no data
Method : other:DIN-Standard 38412 Part 15 (Fish, Acute toxicity test), Method of the
German Standards Institution, Berlin, Germany
Year : 1982
GLP : no
Test substance : other TS: o-Toluidine, purity = 99.6%
Result : An LC50 of > 68.1 mg/l > 100 mg/l was obtained with Probit Analysis.
Generating the geometric mean from 68.1 mg/l and 100 mg/l a LC50 of
82.5 mg/l is to be estimated.
Other results:
100 mg/l < 24h-LC50 < 150 mg/l
68 mg/l < 72h-LC50 < 100 mg/l
By interpolation a 48h-LC50 of 93 mg/l was obtained.
Mortality (%) at the different test concentrations:
Conc. (mg/l) Duration (h)
1 4 24 48 72 96
10.0 0 0 0 0 0 0
14.7 0 0 0 0 0 0
21.5 0 0 0 0 0 0
31.6 0 0 0 0 0 0
46.4 0 0 0 0 0 0
68.1 0 0 0 0 0 0
100 0 0 0 70 100 100
147 0 60 100 100 100 100
215 0 70 100 100 100 100
Test condition : - The test was conducted in 50 l aquarium (60cmx35cmx40cm).
- Recipients were constantly aerated with air (oil free).
- Dilution water had a total hardness of 2.5 mmol/l, a Ca/Mg ions ratio of
4:1 and a Na/K ions ratio of 10:1.
- These conditions were obtained by addition of 294 mg/l CaCl2x2H2O,
123.3 mg/l MgSO4x7H2O, 64.8 mg/l NaHCO3 and 5.8 mg/l KCl.
- 10 fishes were used for the test and were previously adapted during 3
days. Length: 6.1-7.6 cm, Weight: 1.4-3.2 g.
- The following nominal concentrations were tested: 10, 14.7, 21.5, 31.6,
46.4, 68.1, 100, 147 and 215 mg/l.
- The test temperature was 20+/-1°C; pH-value was 7.4+/-0.3, oxygen
concentration varied between 9.2 and 4.1 mg/l during the tests.
Reliability : (2) valid with restrictions
Test procedure in accordance with national standard methods
Flag : Critical study for SIDS endpoint
10.11.2004 (93)
Type : static
Species : Leuciscus idus melanotus (Fish, fresh water)
Exposure period : 48 hour(s)
Unit : mg/l
LC0 : 30
LC50 : 117
LC100 : 178
Limit test :
Analytical monitoring : no
Method : other: no data
Year : 1978
GLP : no data
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