o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
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OECD SIDS o-<strong>TOLUIDINE</strong><br />
5. TOXICITY ID: <strong>95</strong>-<strong>53</strong>-4<br />
DATE: 07.01.2005<br />
Type :<br />
Species : rat<br />
Sex : no data<br />
Strain : no data<br />
Route of admin. : oral unspecified<br />
Exposure period : 2.5 month (no further details given)<br />
Frequency of treatm. : daily<br />
Post exposure period :<br />
Doses : 35 mg/kg b.w./day (no further details given)<br />
Control group : no data specified<br />
Method : other: no data<br />
Year : 1967<br />
GLP : no data<br />
Test substance : other TS: o-toluidine, no data on purity<br />
Result : increased methemoglobin, erythropenie, retikulozytose.<br />
Reliability : (4) not assignable<br />
Insufficient documentation and/or Secondary literature<br />
02.06.2004 (144)<br />
5.5 GENETIC TOXICITY ‘IN VITRO‘<br />
Type : Ames test<br />
System of testing : Salmonella typhimurium TA 98, TA 100, TA 1<strong>53</strong>5, TA 1<strong>53</strong>7<br />
Test concentration : 1) all strains: +/- S9: 0, 100, 333, 1000, 3333, 5000 µg/plate in Ethanol<br />
(abs);<br />
2) TA 98, +S9, + Norharman: 0, 0.10, 0.33, 1.0, 3.3, 10, 33, 100 µg/plate in<br />
Ethanol (abs)<br />
Cycotoxic concentr. : preliminary test: TA100: > 5000 µg/plate (with and without S9-mix) TA98<br />
with S9-mix and norharman: >= 100 ug/plate<br />
Metabolic activation : with and without<br />
Result :<br />
Method : other: Ames, Mutat. Res. 31, 347 (1975); Maron, Mutat. Res.113, 173<br />
(1983) see also freetext ME<br />
Year : 19<strong>95</strong><br />
GLP : yes<br />
Test substance : other TS: o-toluidine, purity 99.<strong>95</strong>%<br />
Method : Preliminary Toxicity test<br />
----Salmonella typhimurium TA100, with and without metabolic activation,<br />
vehicle: ethanol, dose-range: 6.7 - 5000 ug/plate<br />
----Salmonella typhimurium TA98, with S9-mix and norharman, vehicle:<br />
ethanol, dose-range: 6.7 - 5000 ug/plate<br />
Mutagenicity assay:<br />
Plate incorporation methodology<br />
All tester strains were tested with and without metabolic activation.<br />
TA98 additionally treated with S9 + Norharman.<br />
METABOLIC ACTIVATION:<br />
S9 mix liver homogenates from aroclor 1254 induced male Sprague-<br />
Dawley rats<br />
Controls:<br />
negative control: solvent;<br />
positive controls:<br />
with S9: 2-aminoanthracene;<br />
without S9: 2-nitrofluorene; sodium azide; 9-aminoacridine;<br />
<strong>UNEP</strong> PUBLICATIONS 163