o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
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OECD SIDS o-<strong>TOLUIDINE</strong><br />
4. ECOTOXICITY ID: <strong>95</strong>-<strong>53</strong>-4<br />
DATE: 07.01.2005<br />
Test substance : other TS: 99 % purity<br />
Result : CONCENTRATIONS<br />
Recovery rates were always > 90 % with one exception for the lowest<br />
tested concentration at the beginning of the test with 66 % recovery. This is<br />
due to the fact that slight variations for very low concentrations < 0.002<br />
yield usually low recovery values.<br />
EFFECTS<br />
48day LC50 > 0.4 mg/L<br />
21day LC50 = 0.0306 mg/L (<strong>95</strong> % confidence limits: 0.0219-0.0438 mg/L)<br />
21day EC50 = 0.0658 mg/L (<strong>95</strong> % confidence limits: 0.0600-0.0721 mg/L)<br />
21day NOEC = 0.0126 mg/L<br />
21day LOEC = 0.040 mg/L<br />
CUMULATIVE NUMBER OF DEAD PARENTAL DAPHNIA AND THE<br />
MORTALITY AFTER 21 DAYS<br />
measured con- number of mortality<br />
centration (mg/L) dead parent %<br />
---------------------------------------------------------------<br />
control 1 2.5<br />
0.00126 2 5.0<br />
0.0040 0 0<br />
0.0126 1 2.5<br />
0.040 34 85.0<br />
0.126 39 97.5<br />
0.40 40 100<br />
---------------------------------------------------------------<br />
MONITORING DATA<br />
water temperature: 19.8 - 20.4 °C<br />
dissolved oxygen: 8.4 - 8.8 mg/L<br />
(Saturated concentration at 20 °C is 8.84 mg/L)<br />
pH: 7.37 - 7.73<br />
Test condition : TEST SYSTEM<br />
concentrations tested (nominal): 0 (control), 0.00126, 0.0040, 0.0126,<br />
0.040, 0.126, 0.40 mg/L<br />
number of replicate: 4<br />
number of daphnid per replicate: 10<br />
water temperature: 20 +- 1 °C<br />
temperature, oxygen-content and pH were measured at 0, 4, 8, 11, 15, 18,<br />
21 days<br />
renewal of test solution continuously (flow-through system)<br />
photo period: 16hr-8hr light-dark cycle by room light<br />
test parameter: number of dead daphnia, and number of juveniles<br />
produced per adult<br />
analytical monitoring: HPLC at 0, 4, 7, 14, 21 days<br />
Reliability : (1) valid without restriction<br />
GLP guideline study<br />
Flag : Critical study for SIDS endpoint<br />
15.11.2004 (127)<br />
Species : Daphnia magna (Crustacea)<br />
Endpoint : other: immobilization, population growth, and length<br />
Exposure period : 21 day(s)<br />
Unit : mg/l<br />
NOEC : .032<br />
EC50 : 2.2<br />
LRCT : .1<br />
Analytical monitoring : no<br />
Method : other: NEN 6502: Determination of chronic toxicity to Daphnia magna<br />
(1980)<br />
<strong>UNEP</strong> PUBLICATIONS 125
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