o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

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OECD SIDS o-TOLUIDINE 4. ECOTOXICITY ID: 95-53-4 DATE: 07.01.2005 120 Flag : Critical study for SIDS endpoint 10.11.2004 (115) (111) (113) Type : aquatic Species : Tetrahymena pyriformis (Protozoa) Exposure period : 40 hour(s) Unit : mg/l EC50 : ca. 155 Analytical monitoring : no Method : other: Population growth impairment assay Year : 1999 GLP : no data Test substance : other TS: o-Toluidine, purity > 95 % Method : Test was performed according to the method described by Schultz TW (1997). TETRATOX: Tetrahymena pyriformis population growth impairment endpoint. A surrogate for fish lethality. Toxicol. Methods 7, 289-309. - The test was conducted in foam-stoppered 250 ml Erlenmeyer flasks containing 50 ml of sterile, semidefined proteose-peptone-based medium. - Test culture was inoculated with log-growth-phase culture. Initial density varied from 1000 to 5000 cells/ml. - The temperature was maintained at 27+/-1 °C. Medium was buffered to pH 7.4 prior to sterilization. - The assay was conducted as a 40 h static test. Result : Result was given as log 1/IGC50 = -0.16, IGC50 in mM. IGC50 = 50 % growth inhibition concentration Test condition : 200 substances were tested to derive QSAR. Test was performed using the freshwater ciliate Tetrahymena pyriformis (Strain GL-C) The test conditions were as follows: - non-neutralised, allow for 8-9 cell cycles in control cultures. - The pH of the test media was 7.3 and was not controlled during the test. Prior to testing in duplicate for three replicates, the compound was tested in a range finding test. Test replicates consisted of 6 to 8 concentrations with duplicate flasks of each concentration. - The endpoint population density was measured spectrophotometrically at 540 nm. Test substance : The stock solution was presumably dissolved in < 7.5 ml/l DMSO (dimethlysulfoxide). Reliability : (2) valid with restrictions Basic data given Flag : Critical study for SIDS endpoint 11.02.2004 (34) (116) (117) Type : aquatic Species : Tetrahymena pyriformis (Protozoa) Exposure period : 24 hour(s) Unit : mg/l EC50 : = 520 Analytical monitoring : no Method : other: cell multiplication inhibition test Year : 1985 GLP : no data Test substance : other TS: o-Toluidine, analytical grade Method : - 2 different counting methods were used. One used a microscope with a specially prepared glass slide which contained a depression (20x50 mm) that could hold 20 cells. A quantity of cultured medium was dropped onto the glass slide and an adequate amount of 3.7 % formaline and 0.7 % CaCl2 was added to kill the cells. The count was repeated 3 times and UNEP PUBLICATIONS
OECD SIDS o-TOLUIDINE 4. ECOTOXICITY ID: 95-53-4 DATE: 07.01.2005 the mean value of the number of cells was recorded. The other method used a Coulter counter (Model Zb: Nikkaki). The samples were diluted to 50 % with Isotone. The count was repeated twice and the mean value recorded. Test condition : - Tetrahymena pyriformis was preserved in a sterile medium (of 2 % proteose peptone at a temperature of 20 °C) which was renewed at 2-4 week intervals. - Tetrahymena pyriformis was pre-cultured at a temperature of 30 °C for 24 h. - Stock solution of test substance was added to the sterile medium to provide a concentration ratio of 1.8 in 10 ml of 2 % proteose peptone. - The solutions were then inoculated with 0.2 ml of the pre-cultured T. pyriformis and cultivated at a temperature of 30 °C for 24 h without agitation. Test substance : The stock solution was presumably dissolved in < 5 ml/l DMSO (dimethlysulfoxide). Reliability : (2) valid with restrictions Study meets generally accepted scientific principles Flag : Critical study for SIDS endpoint 11.02.2004 (118) Type : aquatic Species : Uronema parduzci (Protozoa) Exposure period : 20 hour(s) Unit : mg/l EC5 : = 21 Analytical monitoring : no Method : other: cell multiplication inhibition test according to Bringmann (1978) Year : 1980 GLP : no Test substance : other TS: o-Toluidine, purity is not specified Method : Bringmann G (1978). Bestimmung der biologischen Schadwirkung wassergefährdender Stoffe gegen Protozoen. I.Bakterienfressende Flagellaten (Modellorganismus: Entosiphon Sulcatum Stein). Z. Wasser u. Abwasser-Forsch.11, 210-215. Result : Reported TT (Toxicity Threshold) is comparable to EC5; value refers to nominal TS concentration Test condition : Temperature = 25 °C; initial pH = 6.9 (adjusted) Reliability : (2) valid with restrictions Test procedure in accordance with generally accepted scientific standards and described in sufficient detail Flag : Critical study for SIDS endpoint 29.01.2004 (119) (120) Type : aquatic Species : Entosiphon sulcatum (Protozoa) Exposure period : 72 hour(s) Unit : mg/l EC5 : = 76 Analytical monitoring : no Method : other: cell multiplication inhibition test according to Bringmann (1978) Year : 1978 GLP : no Test substance : other TS: o-Toluidine, purity is not specified Method : Bringmann G (1978). Bestimmung der biologischen Schadwirkung wassergefährdender Stoffe gegen Protozoen. I.Bakterienfressende Flagellaten (Modellorganismus: Entosiphon Sulcatum Stein). Z. Wasser u. Abwasser-Forsch.11, 210-215. Result : Reported TT (Toxicity Threshold) is comparable to EC5; value refers to UNEP PUBLICATIONS 121
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OECD SIDS o-TOLUIDINE FOREWORD INTR
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OECD SIDS o-TOLUIDINE 11. Comments:
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OECD SIDS o-TOLUIDINE and transitio
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OECD SIDS o-TOLUIDINE 6 REFERENCES
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OECD SIDS o-TOLUIDINE Bringmann G,
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OECD SIDS o-TOLUIDINE Hecht SS, El-
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OECD SIDS o-TOLUIDINE phase extract
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OECD SIDS o-TOLUIDINE Stark M (1892
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OECD SIDS o-TOLUIDINE I U C L I D D
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o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

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