o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
5. TOXICITY ID: 95-53-4
DATE: 07.01.2005
.......................percent of dose
Liver...........0.13.... ..0.12.... ....0.34........0.31
Kidney........0.015.....0.017.......0.06........0.05
Lung...........0.004.....0.003.......0.02........0.01
Spleen........0.002.....0.002.......0.04........0.04
Colon...........-----.... -----....... 0.004......0.006
Bladder.......0.0004....0.0003....0.01........0.02
METABOLITES:
The results shown that N-acetylation and hydroxylation at the 4 position of
o-toluidine are major metabolic pathways in rats. Minor pathways include
hydroxylation at the 6 position, oxidation of the methyl group and oxidation
of the amino group. Sulphate conjugates predominate over glucuronides by
a ratio of 6:1.
--detected substances in the urine:
o-toluidine, azoxytoluene, o-nitrosotoluene, N-acetyl-o-toluidine, N-acetylo-aminobenzyl
alcohol, 4-amino-m-cresol, N-acety-4-amino-m-cresol,
anthranilic acid, N-acetylanthranilic acid, 2-amino-m-cresol and unidentified
substances.
--substances which were excreted via feces or via exhailed air were not
mentioned
Test condition : TYP: Metabolism and Distribution
TEST ORGANISMS:
- Animals: male F344 rats
- Weight at study initiation: 250-300g
ADMINISTRATION and EXAMINATIONS:
- animals were subcutan injected with a single dose of either 50 mg or 400
mg/kg ortho-[methyl-14C]-toluidine hydrochloride/kg bw as a suspension in
0.9% NaCl
-Immediately after injection animals were placed in metabolism cages.
--The expired air was pased through two gas washers in series.
--Urine and feces samples were cooled collected for time periods of 24h
and 48h.
-Animals were killed 48 h after injection and selected organs were removed
and homogenized.
Metabolites were identified using paper electrophoresis colum
cromatography, thin layer chromatography, high pressure liquid
chromatography and gas chromatography-mass spectrometry.
Test substance : ortho-[methyl-14C]-toluidine hydrochloride and
non labelled ortho-toluidine hydrochloride
Reliability : (1) valid without restriction
Flag : Critical study for SIDS endpoint
13.12.2004 (129) (130)
In Vitro/in vivo : In vivo
Type : Distribution
Species
Number of animals
: rat
Males : 4
Females
Doses
:
Males : 500 mg/kg bw in corn oil/methanol (8:2)
Females :
Vehicle : other: corn oil/methanol (8:2)
Route of administration : other: single application by gavage
Exposure time : 72 hour(s)
Product type guidance :
Decision on results on acute tox. tests :
Adverse effects on prolonged exposure :
130
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