o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
5. TOXICITY ID: 95-53-4
DATE: 07.01.2005
Evaluation of Short-Term Tests for Cancerogens Progress in Mutation
Research Vol 1. Edited by Frederick J. Serres and John Ashby, 1981
CHEMICALS:
42 coded chemicals were investigated in short-term tests for
cancerogenicity in different laboratories
It should be suggested that only 100%-pure chemicals should be used
when evaluating a test system.
---In the present work the compound with code no 41 is declared as otoluidine
hydrochloride, whereas in an the overall summary of this program
code no. 41 is declared as o-toluidine.
METABOLIC ACTIVATION:
S9 mix - liver homogenates from with Aroclor 1254 induced rats
CONCENTRATIONS:
The concentration range in testing was determined using E.coli rec-tester
strains. An initial concentration of 500 µl/ml was examined. If the initial
concentration of 500 µl/ml was toxic, serial diluted samples were examined
until the toxic limit was determined. If the initial concentration was not toxic,
higher concentrations were examined until the toxic limit was obtained
(highest concentration examined 1.0 g/ml). Dose-response curves were
obtained from the toxic limit to level of no effect or at least at tenfold
dilution.
Reliability : (4) not assignable
Method-Evaluation, insufficient documentation, unclear use: o-toluidine or
o-toluidine hydrochloride
15.07.2004 (152) (185)
Type : Ames test
System of testing : Salmonella typhimurium TA 98, TA 100, TA 1535, TA 1537, TA 1538
Test concentration : 0.32, 1.0, 3.2, 10 µl/plate
Cycotoxic concentr. : preliminary cytotoxicity test
Metabolic activation : with and without
Result : negative
Method : other: according to Ames et al., Mutat. Res. 31, 347 (1975)
Year : 1981
GLP : no data
Test substance : other TS: see freetext TC
Result : o-toluidine was tested negative. An evaluation in TA1538 revertant colonies
at 3.2µl/plate was not reproduced in a repeat assay.
Test condition : This study is part of a International Collaborative Program, published as:
Evaluation of Short-Term Tests for Cancerogens Progress in Mutation
Research Vol 1. Edited by Frederick J. Serres and John Ashby, 1981
CHEMICALS:
42 coded chemicals were investigated in short-term tests for
cancerogenicity in different laboratories
It should be suggested that only 100%-pure chemicals should be used
when evaluating a test system.
---In the present work the compound with code no 41 is declared as otoluidine
hydrochloride, whereas in an the overall summary of this program
code no. 41 is declared as o-toluidine.
METABOLIC ACTIVATION:
S9 mix - liver homogenates from with Aroclor 1254 induced male Wistar
rats
Reliability : (2) valid with restrictions
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