o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
5. TOXICITY ID: 95-53-4
DATE: 07.01.2005
172
Type : Chromosomal aberration test
System of testing : Chinese hamster lung (CHL) fibroblast cells
Test concentration : 0, 1000, 1250, 1500 µg/ml in DMSO
Cycotoxic concentr. : preliminary test, 50% growth inhibition at 1500 µg/ml
Metabolic activation : with and without
Result : positive
Method : other: Chromosomal aberration test according Ishidate and Odashima
1977
Year : 1985
GLP : no data
Test substance : other TS: o-toluidine, no data on purity
Result : o-toluidine induced structural aberrations with or without metabolic
activation. Minimum effective dose was 1000 µg/ml. Toluidine also induced
polyploid cells with relatively low frequencies.
Test condition : Cells were treated 24 and 48 hours with test chemical at least 3 doses
including the 50% cell growth inhibition dose.
Metabolic activation: S9 mix liver homogenates from PCB induced rats
Controls: solvent control
Positive controls: N'-methyl-N'-nitro-N-nitrosoguanidine; mitomycin C;
benz[a]pyrene; dimethylnitrosamine
Reliability : (2) valid with restrictions
no data on purity and no information on GLP
Flag : Critical study for SIDS endpoint
21.05.2004 (178) (180) (181)
Type : Sister chromatid exchange assay
System of testing : Chinese hamster ovary (CHO) cells
Test concentration : -S9-mix: (1) 0, 1.6, 5, 16, 50, 160, 500 µg/ml; (2)0, 25, 50, 100, 250, 500
µg/ml
+S9-mix: (1) 0, 16, 50, 160, 500, 1600, 5000 µg/ml; (2) 0, 500, 700, 1000,
1500, 2000 µg/ml
Cycotoxic concentr. : -S9-mix: > 500 µg/ml; +S9-mix: >= 2000 µg/ml
Metabolic activation : with and without
Result : positive
Method : other: see freetext TC
Year : 1985
GLP : no data
Test substance : other TS: o-toluidine, no data on purity
Result : o-Toluidin was tested positive with and without metabolic activation
The positive controls were functional.
Test condition : Incubation time:
without metabolic activation:
The total incubation time was 26 hours: 2 hours with TS alone, adding
BRdU for 24 hours, adding colcemid the last 2-3 hours
with metabolic activation:
Cells were incubated for 2 hours in the presence of TS, then cells were
wasched and BRdU was added for 26 hourswith colcimid the last 2-3 hours
METABOLIC ACTIVATION:
S9-mix was prepared from liver homogenates from Aroclor 1254 injected
male Sprague-Dawley rats
CONCENTRATIONS:
Chemicals were tested up to a maximum concentration of 5 mg/ml unless
limited by solubility or toxicity.
Controls:
a) negative controls : cells received no treatment
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