o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
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OECD SIDS o-<strong>TOLUIDINE</strong><br />
5. TOXICITY ID: <strong>95</strong>-<strong>53</strong>-4<br />
DATE: 07.01.2005<br />
218<br />
HU/ara-C at 1.70 mM and 4.24 mM<br />
Test condition : in presence (to increase the sensitivity) or in absence of the DNA-repair<br />
inhibitors - hydroxyurea and cytosine arabinoside (HU/araC)<br />
toxicity is measured as cell viability parallel to the comet assay<br />
no data on positive control<br />
Reliability : (2) valid with restrictions<br />
special study<br />
15.07.2004 (297)<br />
Type : other: DNA damage<br />
System of testing : human breast milk cells<br />
Test concentration : 0.85 mM<br />
Cycotoxic concentr. : > 0.85 mM<br />
Metabolic activation : without<br />
Result : positive<br />
Method : other: alkaline single-cell gel electrophoresis (comet) assay according to<br />
Singh et al.,1988, Exp. Cell Res. 175, 184-191<br />
Year : 2000<br />
GLP : no data<br />
Test substance : other TS: o-toluidine, no data on purity<br />
Result : Comet tail length were significantly increased in the presence of the DNArepair<br />
inhibitors - hydroxyurea and cytosine arabinoside (HU/araC) and in<br />
absence of cytotoxicity (83% of cells viable)<br />
Reliability : (4) not assignable<br />
special study<br />
15.07.2004 (298) (299)<br />
Type : other: DNA damage<br />
System of testing : human and rat epithel cells of the urinary bladder<br />
Test concentration : 8, 16 and 32 mM<br />
Cycotoxic concentr. : > 80 mM<br />
Metabolic activation :<br />
Result : positive<br />
Method : other: alkaline single-cell gel electrophoresis (comet) assay according to<br />
Singh et al.,1988, Exp. Cell Res. 175, 184-191<br />
Year : 2002<br />
GLP : no data<br />
Test substance : other TS: o-toluidine, purity as reagent grade<br />
Result : Significant dose-dependent increases of DNA fragmentation were obtained<br />
in cells from rats at 8, 16 and 32 mM and in cells from humans with<br />
concentrations from 16 and 32 mM.<br />
Test condition : Primary human and rat epithel cells of urinary bladder were incubated with<br />
test substance over a time period of 20 hours.<br />
DNA damge was quantified by the increase of tail moment, that was<br />
defined as the product of the tail length and the amount of DNA in the tail<br />
(Olive et al., 1990).<br />
Statistical analysis was performedby the use of analysis of variance<br />
followed by Dunnet's test.<br />
solvent: DMSO<br />
Reliability : (2) valid with restrictions<br />
meets scientific principles<br />
15.07.2004 (300)<br />
Type : other: DNA single-strand breaks<br />
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