o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
4. ECOTOXICITY ID: 95-53-4
DATE: 07.01.2005
4.1 ACUTE/PROLONGED TOXICITY TO FISH
104
Type : semistatic
Species : Oryzias latipes (Fish, fresh water)
Exposure period : 96 hour(s)
Unit : mg/l
LC0 : 88.9
LC50 : 151
LC100 : 200
Limit test : no
Analytical monitoring : yes
Method : OECD Guide-line 203 "Fish, Acute Toxicity Test"
Year : 1996
GLP : yes
Test substance : other TS: 99 % purity
Result : CONCENTRATIONS
Recovery rates always > 86 %
EFFECTS (mortality)
48h LC0 = 88.9 mg/L
48h LC50 = 151 mg/L (95 % confidence limits: 88.9-200 mg/L)
48h LC100 = 200 mg/L
MORTALITY
nominal concent- cumulative number of dead (%)
ration (mg/L) 24hr 48hr 72hr 96hr
-------------------------------------------------------------------------------------
control 0 (0) 0 (0) 0 (0) 0 (0)
59.3 0 (0) 0 (0) 0 (0) 0 (0)
88.9 0 (0) 0 (0) 0 (0) 0 (0)
133 0 (0) 0 (0) 2 (20) 2 (20)
200 0 (0) 7 (70) 10 (100) 10 (100)
300 0 (0) 10 (100) 10 (100) 10 (100)
-------------------------------------------------------------------------------------
MONITORING DATA
water temperature: 23.1 - 24.0 °C
dissolved oxygen: 6.9 - 8.4 mg/L
(Saturated concentration at 24 °C is 8.25 mg/L)
pH: 7.40 - 7.74
Test condition : TEST SYSTEM
concentrations tested: 0 (control), 59.3, 88.9, 133, 200, and 300 mg/L
feeding during test: none
number of replicate: 1
number of fish per dose: 10
water temperature: 24 +- 1 °C
temperature, oxygen content and pH were measured every 24 hours
photo period: 16h-8h light-dark cycle by room light
test parameter: mortality, toxic behavior
analysis method: HPLC at 0 and 24 hours
Reliability : (1) valid without restriction
GLP guideline study
Flag : Critical study for SIDS endpoint
15.11.2004 (92)
Type : static
Species : Leuciscus idus (Fish, fresh water)
Exposure period : 96 hour(s)
UNEP PUBLICATIONS