o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
4. ECOTOXICITY ID: 95-53-4
DATE: 07.01.2005
Year : 1986
GLP : no data
Test substance : other TS: o-Toluidine, purity > 99.5%
Method : OECD TG 201 with slight modifications according to Van Leeuwen CJ et al.
(1985). Aquatic toxicological aspects of dithiocarbamates and related
compounds. Short-term tests. Aquat. Toxicol. 7, 145-164.
Test condition : Stock solution of the test substance was prepared in dimethylsulfoxide
(DMSO, Merck, Purity 99%)
Reliability : (2) valid with restrictions
Guideline study without detailed documentation
Flag : Critical study for SIDS endpoint
10.11.2004 (30)
Species : other algae: Scenedesmus obliquus
Endpoint : growth rate
Exposure period : 48 hour(s)
Unit : mg/l
EC50 : = 490
Limit test :
Analytical monitoring : no data
Method : OECD Guide-line 201 "Algae, Growth Inhibition Test"
Year : 2001
GLP : no data
Test substance : other TS: o-Toluidine, purity is not specified
Result : Result was given as log (1/EC50)=2.34, where EC50 is given in
mol/l. Furthermore the EC50 was calculated using QSAR-approach and a
log (1/EC50)=2.58 for o-toluidine was obtained. This result corresponds to
an EC50 of 282 mg/l.
Test condition : - The algae in logarithmic growing period were inoculated into 250 ml
flasks, amounting to 60 ml of culture media, compound and the algae. The
initial algae cell concentration in the test culture was approximately 1E+04
cells/ml.
- The culture was incubated under continuous light at a temperature of
20+/-1 °C for 48 h and the average illumination intensity was about 4000
lux produced by white fluorescent lamp.
- Five concentration gradients were planned before beginning of the test.
Space between gradients was 0.2.
- Growth was monitored by electron microscope.
Reliability : (2) valid with restrictions
Guideline study without detailed documentation
Flag : Critical study for SIDS endpoint
18.06.2004 (33) (108)
Species : Microcystis aeruginosa (Algae, blue, cyanobacteria)
Endpoint : biomass
Exposure period : 8 day(s)
Unit : mg/l
EC3 : = .31
Limit test :
Analytical monitoring : no
Method : other: Cell multiplication inhibition test
Year : 1978
GLP : no
Test substance : other TS: o-Toluidine, purity is not specified
Method : - Before starting the test, the stock solution of o-toluidine was neutralised.
- The solutions with the test concentrations were then prepared in 300 ml
Erlenmeyer flasks.
- The extinction value of the monochromatic radiation (578 nm) of the cell
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