o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
3. ENVIRONMENTAL FATE AND PATHWAYS ID: 95-53-4
DATE: 07.01.2005
90
toluidine system)
Reliability : (2) valid with restrictions
Guideline study with acceptable restrictions
Flag : Critical study for SIDS endpoint
20.06.2004 (70)
Type : aerobic
Inoculum : other: effluent from STP predominantly treating domestic sewage
Concentration : 20 mg/l related to DOC (Dissolved Organic Carbon)
related to
Contact time :
Degradation : 67 - 90 (±) % after 28 day(s)
Result : readily biodegradable
Deg. product :
Method : OECD Guide-line 301 E "Ready biodegradability: Modified OECD
Screening Test"
Year : 1983
GLP : no
Test substance : other TS: o-Toluidine, purity is not specified
Method : Six laboratories took part following the same protocol which specified the
details of the test procedure.
- Tests were carried out using an inoculum prepared from the same source
which was recommended to be the final effluent from a sewage works
treating predominantly domestic sewage.
The concentration stage required was achieved by either membrane
filtration or centrifugation (inoculum density was between 2E+07 and
5E+05 bacteria/ml), although one of the six laboratories used activated
sludge (30 mg/l)
- Reference compound control: aniline (validity criterion: 70 % aniline
degradation by day 14); analysis both by DOC and substance specific
analysis (GC)
Remark : Biodegradation was referred to DOC-elimination.
Result : The days for analysis were 0, 3, 7, 14, 21 and 28 and analysis by both
DOC and by specific analysis (gas chromatography recommended) was
specified.
5 laboratories reported biodegradation of > 90 %. However, one of these
laboratories obtained 0 % after 28 d, although this was considered an
anomalous result, as > 90 % biodegradation after 7 days was measured in
the same experiment. In the sixth laboratory a 67 % rate was observed.
From the results given after 7 and 14 days it can be assumed that in all
experiments the pass level of 70 % was reached within the 10 days
window.
One of the labs presented 2 series of results, as in the first experiment an
extreme low degradation was obtained which was not in accordance with
all other results. The results of the first series were therefore not
considered.
Reliability : (2) valid with restrictions
Test procedure in accordance with standard guideline with acceptable
modifications
Flag : Critical study for SIDS endpoint
15.06.2004 (71)
Type : aerobic
Inoculum : other: effluent from STP predominantly treating domestic sewage
Concentration : 20 mg/l related to DOC (Dissolved Organic Carbon)
related to
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