o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
OECD SIDS o-<strong>TOLUIDINE</strong><br />
5. TOXICITY ID: <strong>95</strong>-<strong>53</strong>-4<br />
DATE: 07.01.2005<br />
206<br />
The positive controls were functional.<br />
Test condition : Treatment Time:<br />
without S9-mix: 48 hours<br />
with S9-mix: 3 hours<br />
Controls:<br />
negative control: solvent (DMSO)<br />
positive control: Dimethylnitrosamin (DMN), Benzopyrene (BP)<br />
METABOLIC ACTIVATION:<br />
S9 mix liver homogenates with phenobarbital and 5,6-benzoflavone<br />
induced rats<br />
Reliability : (2) valid with restrictions<br />
assay system evaluation<br />
15.07.2004 (173) (274)<br />
Type : Mammalian cell gene mutation assay<br />
System of testing : human cell lines:TK6-cells ((thymine kinase locus) and AHH-1-cells<br />
(Hypoxanthine guanine phosphoribosyl transferase locus)<br />
Test concentration : Tk6-cells: -S9-mix: 0, 150, 300, 450, 600, 750 µg/ml; +S9-mix: 0, 450, 900,<br />
1350 µg/ml<br />
AHH-1-cells:-S9-mix: 0, 100, 200, 300, 400, 500 µg/ml;<br />
Cycotoxic concentr. : preliminary cytotoxicity test<br />
Metabolic activation : with and without<br />
Result : positive<br />
Method : other: human lymphoblast gene-locus mutation assay, according Thilly et<br />
al. (1976), Chem.-Biol. Interact 15, 33-50<br />
Year : 1985<br />
GLP : no data<br />
Test substance : other TS: o-toluidine, no data on purity<br />
Method : Preliminary cytotoxicity determination<br />
to define the dose-range<br />
Mutation test:<br />
negative control was the solvent: DMSO<br />
positive controls: 4-nitroquinoline-N-oxide, benzo(a)pyrene<br />
Exposure time:<br />
TK6-cells, -S9-mix: 20 hours (approx. one cell generation)<br />
TK6-cells, +S9-mix: 3 hours<br />
AHH-1-cells (metabolic competent): 28 hours (approx. one cell generation)<br />
Metabolic activation system: Arocolor 1254 activated rat liver cells<br />
Result : o-toluidine was mutagenic in TK6 cells in the presece of S9-mix at 450<br />
µg/ml and higher and in the metabolic competent AHH-1 cells at 300 µg/ml<br />
and higher.<br />
The positive controls were functional.<br />
Reliability : (2) valid with restrictions<br />
assay system evaluation, well documented<br />
15.07.2004 (173)<br />
Type : Mouse lymphoma assay<br />
System of testing : L5178Y TK-/TK+<br />
Test concentration : 0, 0.1, 0.3, 1.0, 3.0, 10 µl/ml<br />
Cycotoxic concentr. : 1 µl/ml = ca. 1mg/µl<br />
Metabolic activation : with and without<br />
Result : negative<br />
Method : other<br />
Year : 1985<br />
GLP : no data<br />
Test substance : other TS: o-toluidine, no data on purity<br />
Result : o-toluidine at a concentration of 2µl/ml or higher for 2h was almost<br />
completely toxic to cells.<br />
No mutagenic effect was observed under any of the test conditions.<br />
<strong>UNEP</strong> PUBLICATIONS
Change language