o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
5. TOXICITY ID: 95-53-4
DATE: 07.01.2005
no clear decision about the TS used
28.01.2004 (337)
Type : other: DNA Single-strand break
Species : mouse
Sex : male
Strain : other: Swiss CD-1
Route of admin. : i.p.
Exposure period : once
Doses : 0, 100 mg/kg bw
Result : positive
Method : other: alkaliche elution according to Cesarone et al., 1979, Anal.
Biochem.100, 188-197
Year : 1980
GLP : no data
Test substance : other TS: o-toluidine, no data on purity
Method : Single i.p. injection of o-toluidine in to male Swiss mice.
4 hours after application isolation of nuclei from liver and kidneys and lysis
on a membrane filter. DNA is eluted using alkaline buffer as function of
molecular weight.
As negative control: solvent
Remark : Single-strand DNA-breaks were observed in liver and kidney nuclei.
Reliability : (2) valid with restrictions
no data on purity of TS, no data on GLP and only one dose used
18.05.2004 (338) (339)
Type : other: DNA binding
Species : rat
Sex : male
Strain : Sprague-Dawley
Route of admin. : i.p.
Exposure period : single administration
Doses : 10, 20, 50, 100 mg/kg bw
Result :
Method : other: 32P post-labelling
Year : 1993
GLP : no data
Test substance : other TS: o-toluidine, no data on purity
Reliability : (4) not assignable
abstract, indufficient documentation
18.05.2004 (340)
Type : other: DNA lesions (Comet assay)
Species : mouse
Sex : male
Strain : other: ddY mice
Route of admin. : gavage
Exposure period : single dose
Doses : 0, 600 mg/kg bw. in olive oil
Result : positive
Method : other: alkaline single cell gel electrophoresis (SCG) assay
Year : 1999
GLP : no data
Test substance : other TS: o-toluidine, purity > 99%
Result : Stomach, liver, lung, urinary bladder, and brain were positive in the SCG
assay.
Test condition : TEST ORGANISMS:
- Age: 7 weeks
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