o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
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OECD SIDS o-<strong>TOLUIDINE</strong><br />
5. TOXICITY ID: <strong>95</strong>-<strong>53</strong>-4<br />
DATE: 07.01.2005<br />
ADMINISTRATION / EXPOSURE<br />
- Duration of test/exposure: 5, 10 or 20 days<br />
- Type of exposure: gavage without vehicle at 25%<br />
of the estimated oral LD50 (900 mg/kg bw, Jacobson 1972)<br />
CLINICAL OBSERVATIONS AND FREQUENCY:<br />
- Clinical signs: daily<br />
- Mortality: daily<br />
- Body weight: at necropsy<br />
ORGANS EXAMINED AT NECROPSY:<br />
- Organ weights: liver, spleen, thyroid, urinary bladder<br />
and kidneys<br />
Microscopic: fixed organs liver, spleen, thyroid, urinary bladder, trachea<br />
and esophagus<br />
Bone marrow impressions were made from a cross section of femur<br />
Histopathological evaluation:<br />
scoring system:<br />
0 = no effect - 4 = severe effect<br />
Data presented as means score for each group and sacrifice date: total<br />
score for sesions in each group/number of rats examined for the same<br />
group<br />
STATISTICAL METHODS:<br />
- Analysis of variance,<br />
- Dunnett's procedure<br />
- Fisher's Exact Test<br />
Reliability : (2) valid with restrictions<br />
only one dose group<br />
Flag : Critical study for SIDS endpoint<br />
13.12.2004 (158)<br />
Type : Sub-chronic<br />
Species : rat<br />
Sex : male/female<br />
Strain : Fischer 344<br />
Route of admin. : oral feed<br />
Exposure period : 7 weeks<br />
Frequency of treatm. : daily<br />
Post exposure period : 1 week<br />
Doses : 0, 1000 - 50000 ppm (approximately 0, 75 - 3750 mg/kg bw/d, see also<br />
freetext TC)<br />
Control group : yes, concurrent no treatment<br />
Method : other: Estimation of the maximum tolerated dose to select doses for<br />
cancerogenesis bioassay (see also freetext TC)<br />
Year : 1979<br />
GLP : no data<br />
Test substance : other TS: o-Toluidine hydrochloride, purity > 99%<br />
Result : MORTALITY<br />
- Time of death:<br />
4/5 males and 3/5 females died in the 50000 ppm group by week 3 on<br />
study<br />
TOXIC RESPONSE/EFFECTS BY DOSE LEVEL:<br />
- Clinical signs: no clinical signs reported<br />
- Body weight:<br />
dose dependent reduced up to 42 % of the control<br />
- Gross pathology and Histopathology:<br />
Small amounts of renal and splenic pigmentation were<br />
observed in male and female rats receiving 12500 ppm.<br />
Test condition : ANIMALS<br />
obtained as 4-week -old weanlings , all within 3 days of the same age<br />
housed for 2 weeks before start of the sutdy<br />
<strong>UNEP</strong> PUBLICATIONS 159
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