o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
5. TOXICITY ID: 95-53-4
DATE: 07.01.2005
Type : Micronucleus assay
Species : mouse
Sex : male
Strain : B6C3F1
Route of admin. : i.p.
Exposure period : single administration
Doses : 0, 75, 150, 300 mg/kg bw in DMSO
Result : negative
Method : other: according Preston et al., 1987
Year : 1989
GLP : no data
Test substance : other TS: o-toluidine hydrochloride, no data on purity
Result : ---clinical observations:
Injections of 300 mg/kg bw had a very pronounced sedative effect; within
20-30 min after injection. they were totally immobile and showed minimal
response to physical stimulus.
---PCE production was slightly suppressed.
---Positive control was functional.
Test condition : In preliminary tests the MTD was determined; doses chosen were MTD,
0.5 MTD and 0.25 MTD
TEST ORGANISMS:
- Age: 10 - 16 weeks
- No. of animals per dose: 8
ADMINISTRATION:
- Vehicle: DMSO
- Administration volume: 5 ml/kg body weight
- Duration of test:
animals were killed 24, 48, or 72 h after treatment and
bone marrow samples were taken;
1000 polychromatic erythrocytes were taken from each animal;
determination of the ratio of polychromatic/normochromatic erythrocytes
- Control groups: negative (vehicle) control group and as
positive control 7,12-dimethylbenz[a]anthracene (DMBA) treated with
single dose of 0.5 mg Mitomycin C/kg i.p.
STATISTICAL EVALUATION:
Kastenbaum-Bowmann tables
Reliability : (2) valid with restrictions
individual animal data not given, no information on GLP and substance
purity
Flag : Critical study for SIDS endpoint
18.05.2004 (324)
Type : Micronucleus assay
Species : mouse
Sex : male
Strain : CD-1
Route of admin. : i.p.
Exposure period : see freetext test conditions
Doses : see freetext test conditions
Result : negative
Method : other: micronucleus assay in mouse bone marrow, see freetext TC
Year : 1994
GLP : no data
Test substance : other TS: o-toluidine, no data on purity
Result : (1) single-treatment study:
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