o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
5. TOXICITY ID: 95-53-4
DATE: 07.01.2005
168
The positive control was functional
(2) o-toluidine hydrochloride was tested negative in TA 98 with and without
metabolic activation systems; o-toluidine hydrochloride was tested negative
in TA 100 in the absence of S9-mix and in the presence of 30 % S9-mix
prepared from rat liver but yielded positive results in the presence of 30 %
S9-mix from hamster liver.
The positive controls were functional.
Reliability : (2) valid with restrictions
only 4 strains used, no cytotox. concentration given, no information on GLP
Flag : Critical study for SIDS endpoint
11.08.2004 (171)
Type : HGPRT assay
System of testing : Chinese hamster V 79 cells
Test concentration : Trial 1: see freetext TC Trial 2: see freetext TC
Cycotoxic concentr. : with and without serum: preliminary cytotoxicity study, with different doses
and exposure times with and without S9-mix
Metabolic activation : with and without
Result : negative
Method : other: as described by McMillan and Fox (1979)
Year : 1985
GLP : no data
Test substance : other TS: o-toluidine, no data on purity
Result : Trial 1:
Little evidence of toxicity (except at very high concentrations) was
observed
Trial 2:
o-Toluidine did not induce increases in mutant frequencies over control
levels
Positive controls were functional
Test condition : METHOD:
1. INHIBITION OF COLONY FORMING ABILITY
Cells were exposed to the test substance in the presence and in the
absence of serum and in the presence and absence of S9-mix;
exposure time: 1 hour
doses:
highest non-tox.dose:
with and without serum, +/-S9-mix, 0.1mg/ml;
conc. for 50 % kill:
with Serum, +/-S9-mix: >3mg/ml, without Serum: -S9-mix, 3mg/ml, +S9mix,
2mg/ml
2. MUTATION ASSAY
Thioguanine was used to select for HGPRT-deficient mutants.
Exposure to the testsubstance was for 60 min. in the absence of serum
and in the presence and absence of S9-mix.
Doses tested were chosen to include at least one that induced
measureable cytytotoxicity in colony forming assay and one higher dose.
without serum, +/- S9-mix: 0, 0.25, 0.30, 0.50, 1.00, 2.00 mg/ml
expression time: 5, 8, 12 days
CONTROLS
Positive control
cyclophosphamide and ethylmethansulfonate in the presence and in the
absence of S9 mix, respectively
negative control
Solvent: DMSO
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