o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
3. ENVIRONMENTAL FATE AND PATHWAYS ID: 95-53-4
DATE: 07.01.2005
Inoculum : other: activated sludge, aniline-acclimated
Concentration : 500 mg/l related to Test substance
related to
Contact time :
Degradation : ca. 60 (±) % after 8 day(s)
Result :
Deg. product :
Method : other: Respirometer test
Year : 1960
GLP : no
Test substance : other TS: o-Toluidine, analytical grade
Method : Test in a Warburg respirometer
Result : Oxidation was recorded as mg O2 uptake per liter of the mixture in the
flask. Results were presented in a graph (O2 uptake (mg/l) with the length
of Warburg run (h)). From this graph a degradation percentage could be
determined.
Air oxidation and volatility of the substrates were determined by a 24 h
Warburg run in the absence of microorganisms. Results of this run were
extrapolated to cover time periods up to 192 hours.
Test condition : - The solution of mineral salts used to prepare the substrate solutions and
the activated sludge suspensions for the Warburg runs had the following
composition: 500 mg/l K2HPO4, 325 mg/l (NH4)HPO4, 50 mg/l NaCl, 50
mg/l CaCl2, 25 mg/l MgSO4 and 5 mg/l FeCl3. The solvent was tap water.
- Simulated sludge was prepared by mixing in tap water an aqueous
solution of glucose and Difco nutrient broth and concentrated mixture of the
same salts employed in the diluent.
- Acclimatization of the activated sludge to aniline as sole source of carbon
and energy was carried out in 1500 ml aeration tubes by the batch-feed
method.
- Original source of microorganisms was mixed liquor from the aeration
tank of a municipal treatment plant.
- The microflora was fed and aerated at least 1 h before use in the Warburg
respirometer.
- Incubation was carried out at 20 °C for 120 to 192 hours.
Each flask was set up to contain 2500 mg/l activated sludge solids and 500
mg/l test compound (substrate) in a total volume of 20 ml.
Reliability : (2) valid with restrictions
Basic data given
15.06.2004 (78)
Type : aerobic
Inoculum : other: suspension of Niagara silt loam
Concentration : 25 mg/l related to Test substance
related to
Contact time :
Degradation : 100 (±) % after 64 day(s)
Result : other: ring cleavage observed
Deg. product :
Method : other: photometrical measurement of ring cleavage; method according to
Alexander M & Aleem MIH (1961). J. Agr. Food Chem. 9, 44.
Year : 1961
GLP : no
Test substance : other TS: o-Toluidine, purity is not specified
Remark : Study design is suitable to derive some general conclusions on the
biodegradability but not to examine the biodegradability of individual
compounds in detail.
Test condition : - Nutrient solution contained inorganic nutrients and test substance as the
sole carbon source.
- 1 ml of a 1 % suspension of Niagara silt loam was added to closed bottle
UNEP PUBLICATIONS 95