o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
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OECD SIDS o-<strong>TOLUIDINE</strong><br />
5. TOXICITY ID: <strong>95</strong>-<strong>53</strong>-4<br />
DATE: 07.01.2005<br />
182<br />
Test condition : two trials were reported:<br />
1. Ames test in vitro<br />
TS: o-toluidine, no name of the solvent but used as negative control,<br />
positive controls not mentioned, performed with and without S9-mix<br />
2. Ames test of Urine extract<br />
300 mg/kg bw o-toluidiene was fed once to male Sprague-Dawley rats (8-<br />
12 animals), urine was collected over 24 hours and extracts were prepared<br />
and used for the Ames test in the presence and in the abbsence of S9-mix.<br />
Urine extract from rats to which no chemical was given served as negative<br />
control<br />
Metabolic activation:<br />
S9 mix was prepared from male Sprague-Dawley rat<br />
livers treated with polychlorinated biphenyl.<br />
Reliability : (4) not assignable<br />
Insufficient documentation: no positive controls, only 2 strains used, solvent<br />
not mentioned no data on purity of Ts and not performed under GLP<br />
15.07.2004 (200)<br />
Type : Ames test<br />
System of testing : Salmonella typhimurium TA 98, TA 100, TA 1<strong>53</strong>5<br />
Test concentration :<br />
Cycotoxic concentr. :<br />
Metabolic activation : with and without<br />
Result : negative<br />
Method : other: according to Ames et al., Mutat. Res. 31, 347 (1975)<br />
Year : 1980<br />
GLP : no data<br />
Test substance : other TS: o-toluidine, purity: 97-99 %<br />
Test condition : METABOLIC ACTIVATION:<br />
S9 mix liver homogenates from Aroclor 1254 induced Sprague-Dawley rats<br />
SOLUTION in DMSO<br />
Reliability : (4) not assignable<br />
documentation insufficient for assessment, no data on concentration<br />
15.07.2004 (201)<br />
Type : Ames test<br />
System of testing : Salmonella typhimurium TA 98, TA 100, TA 1<strong>53</strong>7<br />
Test concentration : without S9 and with S9-mix and norharman: 50, 100, 500, 1000 µg/plate in<br />
DMSO<br />
Cycotoxic concentr. : no data<br />
Metabolic activation : with and without<br />
Result : positive<br />
Method : other: according to Ames et al., Mutat. Res. 31, 347 (1975) : preincubation<br />
methodology, solvent control, no positive control<br />
Year : 1981<br />
GLP : no data<br />
Test substance : other TS: o-toluidine hydrochloride<br />
Remark : positive only in presence of norharman and S9-mix<br />
Reliability : (4) not assignable<br />
test description insufficient for assessment, in presence of co-mutagen<br />
norharman, 3 strains only<br />
15.07.2004 (202)<br />
Type : Ames test<br />
System of testing : Salmonella typhimurium TA 1<strong>53</strong>8<br />
Test concentration : 0, 50, 100 ug/plate in DMSO<br />
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