o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
5. TOXICITY ID: 95-53-4
DATE: 07.01.2005
toluidine as corn oil solution.
Urines were collected over a time period of 6 hours.
All urines were analysed within 4 hours of collection using highperformance
liquid chromatography with electrochemical detection.
Reliability : (4) not assignable
special sudy
09.06.2004 (133)
In Vitro/in vivo : In vivo
Type : Toxicokinetics
Species : rat
Number of animals
Males : 6
Females :
Doses
Males : 0, 75 mg/kg bw in sunflower oil
Females :
Vehicle : other: sunflower oil
Route of administration : i.p.
Exposure time : 3 day(s)
Product type guidance :
Decision on results on acute tox. tests :
Adverse effects on prolonged exposure :
Half-lives : 1 st :
2 nd :
3 rd :
Toxic behaviour :
Deg. product :
Method : other: see RM and TC
Year : 1984
GLP : no
Test substance : other TS: o-toluidine, purity: 99.5%
Remark : Determination of metabolizing enzymes in rat liver , kidneys and lung.
Result : Effects on enzym-activities by organ (significant changes versus resp.
controls)
-LIVER:
--Cytochrome b5: 0.545 versus 0.447 nmol x mg prot[exp.-1]
--NADPH cytochrome c reductase: 201.3 versus 165.8 nmol x mg
prot[exp.-1] x min[exp.-1]
--AHH: 654 versus 295 pmol x mg protein[exp.-1] x min[exp.-1]
-Ratio of Glutathione-S-transferase to AHH activities:
1926 versus 3969 nmol x mg prot[exp.-1] x min[exp.-1]
-Ratio of Epoxide Hydrolase to AHH activities:
1.85 versus 3.35 nmol x mg prot[exp.-1] x min[exp.-1]
- KIDNEYS:
--- AHH: 70.35 versus 2.91 pmol x mg protein[exp.-1] x min[exp.-1]
-Ratio of Glutathione-S-transferase to AHH activities:
2840 versus 63780 nmol x mg prot[exp.-1] x min[exp.-1]
-Ratio of Epoxide Hydrolase to AHH activities:
1.24 versus 34.02 nmol x mg prot[exp.-1] x min[exp.-1]
- LUNGS:
--- AHH: 9.49 versus 4.75 pmol x mg protein[exp.-1] x min[exp.-1]
-Ratio of Glutathione-S-transferase to AHH activities:
5184 versus 12484 nmol x mg prot[exp.-1] x min[exp.-1]
-Ratio of Epoxide Hydrolase to AHH activities:
4.00 versus 9.89 nmol x mg prot[exp.-1] x min[exp.-1]
Test condition : TEST ORGANISMS:
UNEP PUBLICATIONS 133