o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
5. TOXICITY ID: 95-53-4
DATE: 07.01.2005
15.07.2004 (296) (280)
Type : Unscheduled DNA synthesis
System of testing : rat hepatocytes
Test concentration : no data
Cycotoxic concentr. :
Metabolic activation : no data
Result : positive
Method : other: urine method
Year : 1981
GLP : no data
Test substance : other TS: o-toluidine, see freetext RM
Remark : Substance administered to rats, urine tested
Reliability : (4) not assignable
Insufficient documentation
15.07.2004 (219) (214)
Type : other: DNA damage
System of testing : Chinese hamster lung (V79) cells
Test concentration : 0.3, 1, 3, 10 mM in DMSO
Cycotoxic concentr. : no data
Metabolic activation : with
Result : negative
Method : other: alkaline elution method according to Swenberg et al., 1976, Biochem
Biophys Res Commun 72, 732
Year : 1980
GLP : no data
Test substance : other TS: o-toluidine, purity: 97-99 %
Test condition : Incubation time:
2 hours
METABOLIC ACTIVATION:
S9 mix liver homogenates from Aroclor 1254 induced rats
Solvent: DMSO
Controls
no data of positive and negative controls
Reliability : (4) not assignable
no data on positive controls, only tested in the presence of S9-mix
15.07.2004 (201)
Type : other: DNA damage
System of testing : metabolically-competent human MCL-5 cells
Test concentration : 0, 0.85, 1.70, 4.24 mM
Cycotoxic concentr. : > 4.24 mM
Metabolic activation : without
Result : positive
Method : other: alkaline single-cell gel electrophoresis (comet) assay according to
Singh et al.,1988, Exp. Cell Res. 175, 184-191
Year : 1999
GLP : no data
Test substance : other TS: o-toluidine, purity: analytical grade
Result : Viabilty of the cells: control, lowest to highest conc.
---in the absence of HU/ara-C:
99 %, 99 % to 95 %
----in the presence of HU/ara-C:
99 %, 97 % to 89 %
Median comet tail length
significant increased over the background control in the presence of
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