o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
5. TOXICITY ID: 95-53-4
DATE: 07.01.2005
258
Doses : 0.025, 0.25, 25, 250 µg/ml culture medium
Result : positive
Control group :
Method : other: according Styles, 1977, Br. J. Cancer 36, 558
Year : 1981
GLP : no data
Test substance : other TS: see freetext TC
Test condition : This study is part of a International Collaborative Program, published as:
Evaluation of Short-Term Tests for Cancerogens Progress in Mutation
Research Vol 1. Edited by Frederick J. Serres and John Ashby, 1981
CHEMICALS:
42 coded chemicals were investigated in short-term tests for
cancerogenicity in different laboratories
It should be suggested that only 100%-pure chemicals should be used
when evaluating a test system.
In the present work the compound with code no 41 is declared as otoluidine
hydrochloride, whereas in the overall summary of this program
code no. 41 is declared as o-toluidine.
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Tests were performed with and without exogenous metabolic activation
(liver cells from with Aroclor induced male rats S9 mix).
Cytotoxicity determinations were performed.
Reliability : (2) valid with restrictions
no further information at exposure period, special study
01.12.2003 (152) (374)
Species : other: hamster; mammalian cell transformation assay
Sex :
Strain : other: Syrian hamster embryo cells
Route of admin. :
Exposure period :
Frequency of treatm. :
Post exposure period :
Doses : 0, 0.1, 1.0, 10, 100, 500 µg/ml culture medium
Result : positive
Control group : other: yes, concurrent vehicle(DMSO) and historical
Method : other: according Berwald and Sachs, 1965, J. Natl. Cancer Inst. 35, 641-
661
Year : 1985
GLP : no data
Test substance : other TS: no data on purity
Result : o-toluidine was tested positive, the highest frequency of transformed
colonies 0.26% were found at a dose of 1 µg/ml.
(historical control: 0.006 %; concurrent control: 0.014)
Test condition : Chemicals were administered to the SHE cells in dishes.
After an incubating period of 7 days the cells were washed free of the
chemicals and the colonies were fixed and stained and scored for cloning
efficiency and morphological transformation for criteria described by Barrett
and Ts's, 1978.
Tests were performed without exogenous metabolic activation
Negative controls with DMSO
Reliability : (2) valid with restrictions
no positive controls reported, no data on purity of TS, results reported in
brief
25.05.2004 (375)
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