o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
3. ENVIRONMENTAL FATE AND PATHWAYS ID: 95-53-4
DATE: 07.01.2005
GLP : no
Test substance : other TS: o-Toluidine, purity is not specified
Method : - 2.0 l of the sample water were added to 0.5 l of activated sludge and
aeration of the mixed liquor was started.
- After 23 h aeration and 1 h sedimentation 2.0 l of the supernantant
solution were replaced by the sample water.
- The fill and draw system was therefore used to acclimatize the sludge to
the test water, the water in the container was sampled during aeration at
the beginning (0 h) and 24 h later for analysis.
- The oxygen uptake and the decreases in TOC and COD after cultivation
were measured and the biological degradability calculated.
Remark : Acclimatization period: 1 day;
Result : Degradation:
92 % related to COD removal,
83 % related to TOC removal
Test condition : - The conditions in the aeration container were:
MLSS (mixed liquor suspended solids) 2000-3000 mg/l, air flow rate about
150 ml/min, water temperature = 25-30 °C.
Concentration of the water samples was between 100 and 200 mg/l in
terms of COD or about 100 mg/l in terms of the concentration of the test
substance. pH-value was adjusted to neutral; and both nitrogen and
phosphorus were added at 1.3 and 28 mg/l respectively. The salt
concentration of the water samples was adjusted to a chloride ion
concentration of about 5000 mg/l (same level as that of the wastewater
entering the Fukashiba plant).
Reliability : (2) valid with restrictions
Test procedure in accordance with generally accepted scientific standards
and described in sufficient detail
29.01.2004 (75)
Type : aerobic
Inoculum : other bacteria: Rhodococcus rhodochrous (enriched from agricultural soil)
Concentration : 400 mg/l related to Test substance
related to
Contact time :
Degradation : = 100 (±) % after 4 day(s)
Result :
Deg. product :
Method : other
Year : 1984
GLP : no
Test substance : other TS: o-Toluidine, purity is not specified
Deg. products :
3-methylcatechol
Method : A Gram-positive bacterium (Rhodococcus rhodochrous) with the ability to
utilize o-toluidine as sole source of carbon and nitrogen was isolated from
soil.
Result : In mineral salts medium containing (NH4)2SO4, complete removal of otoluidine
(400 mg/l) was achieved after 2 days of incubation. Using a
mineral salts medium without (NH4)2SO4, o-toluidine served as sole
source of carbon, nitrogen and energy and was completely degraded after
4 days.
In resting-cell experiments 3-methylcatechol was identified as primary
metabolite, which was found to be further degraded to 2-hydroxy-6oxohepta-2,4-dienoic
acid.
Test condition : - Enrichment: A flower-pot was filled with 150 g soil and 0.25 g of otoluidine.
The probe was kept at 30 °C with regular moistening. When otoluidine
was degraded, as tested by thin-layer chromatography (TLC),
UNEP PUBLICATIONS 93