o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
5. TOXICITY ID: 95-53-4
DATE: 07.01.2005
and the colonies were fixed and stained for counting and examination.
Tests were performed without exogenous metabolic activation
Positive controls with benzo[a]pyrene
Negative (solvent) controls with DMSO
Reliability : (2) valid with restrictions
no data on GLP and on the purity of the TS, result description only in brief
28.11.2003 (370) (372)
Species : other: hamster; mammalian cell transformation assay
Sex :
Strain : other: Baby hamster kidney cells (BHK 21 C13/HRC 1)
Route of admin. :
Exposure period :
Frequency of treatm. :
Post exposure period :
Doses :
Result : positive
Control group :
Method : other: according Styles, 1977, Br. J. Cancer 36, 558
Year : 1981
GLP : no data
Test substance : other TS: see freetext TC
Result : In the absence of S9 mix 50% cytotoxicity was reported at 606 µg/ml and in
presence of S9 at 362 µg/ml.
Counted spontaneous transformations without S9 mix were 7 and 22 with
S9 mix.
Test condition : This study is part of a International Collaborative Program, published as:
Evaluation of Short-Term Tests for Cancerogens Progress in Mutation
Research Vol 1. Edited by Frederick J. Serres and John Ashby, 1981
CHEMICALS:
42 coded chemicals were investigated in short-term tests for
cancerogenicity in different laboratories
It should be suggested that only 100%-pure chemicals should be used
when evaluating a test system.
In the present work the compound with code no 41 is declared as otoluidine
hydrochloride, whereas in an overall summary of this program
code no. 41 is declared as o-toluidine.
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Chemicals were administered to cells in petri-dishes. After incubating
period of 21 days the cells were washed free of the chemicals and the
colonies were fixed and stained for counting and examination.
Tests were performed with and without exogenous metabolic activation
(liver cells from with Aroclor induced male rats S9 mix).
Cytotoxicity determinations were performed.
Reliability : (2) valid with restrictions
no further information on doses, special study
25.05.2004 (373) (152)
Species : other: hamster; mammalian cell transformation assay
Sex :
Strain : other: Baby hamster kidney cells (BHK)
Route of admin. :
Exposure period :
Frequency of treatm. :
Post exposure period :
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