o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
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OECD SIDS o-<strong>TOLUIDINE</strong><br />
5. TOXICITY ID: <strong>95</strong>-<strong>53</strong>-4<br />
DATE: 07.01.2005<br />
160<br />
- Number of animals: 5 per sex and dose<br />
ANIMAL MAINTENANCE<br />
- water provided ad libitum<br />
- air of the animal room at a temperature of 22-24°C, relative humidity: 45-<br />
55%, room air changed 15 times per hour<br />
- fluorescence lightening : 12 hour per day cycle<br />
ADMINISTRATION / EXPOSURE<br />
- Diet: presterilized Wayne Sterilizable Lab Meal with 4%<br />
fat, provided at libitum<br />
- Doses: 0-1000-2000-3000-4000-6000-6200-12500-25000 and<br />
50000 ppm (approximately 0-75-150-225-300-450-465-938-1875 and<br />
3750 mg/kg bw), prepared fresh every 1 to 1-1/2 weeks<br />
CLINICAL OBSERVATIONS AND FREQUENCY:<br />
- Clinical signs: daily<br />
- Mortality: twice daily<br />
- Body weight: twice per week<br />
NECROPSY:<br />
at the end of study, gross examination of spleen and kidneys<br />
Evaluation of the results: Ten percent depression in body weight was taken<br />
as the major criterion for the estimation of maximum tolerated dose<br />
Conclusion : Based on the data the low and high dose for a carcinogenesis bioassay<br />
were set on 3000 and 6000 ppm.<br />
Reliability : (2) valid with restrictions<br />
dose finding study for cancerogenesis bioassay (see chapter 5.7)<br />
03.06.2004 (159)<br />
Type : Sub-chronic<br />
Species : mouse<br />
Sex : male/female<br />
Strain : B6C3F1<br />
Route of admin. : oral feed<br />
Exposure period : 7 weeks<br />
Frequency of treatm. : continuous<br />
Post exposure period : 1 week<br />
Doses : 0, 3100 - 50000 ppm (approximately 0, 465-7500 mg/kg bw/d see also<br />
freetext TC)<br />
Control group : yes, concurrent no treatment<br />
Method : other: dose finding study for cancerogenesis bioassay<br />
Year : 1979<br />
GLP : no data<br />
Test substance : other TS: o-Toluidine hydrochloride, purity > 99%<br />
Result : MORTALITY<br />
- no animal died during the study<br />
TOXIC RESPONSE/EFFECTS BY DOSE LEVEL:<br />
- Clinical signs:<br />
no clinical signs reported<br />
- Body weight:<br />
dose dependent reduced up to 63 % of the control<br />
- Gross pathology and Histopathology:<br />
50000 ppm:<br />
spleen - red pulp with pigment deposition, apparently<br />
lipofuscin,<br />
kidneys - similar pigmentation in trace up to small<br />
amounts in the tubular epithelium<br />
liver - trace amounts of pigment in Kupffer's cells of the<br />
hepatic sinusoids in both sex<br />
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