o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
5. TOXICITY ID: 95-53-4
DATE: 07.01.2005
Species : other: mammalian cell transformation assay
Sex :
Strain : other: Syrian hamster embryo (SHE) cells
Route of admin. :
Exposure period :
Frequency of treatm. :
Post exposure period :
Doses : Lab A: 0, 200, 300, 400, 500,600,700, 800, 900, 1000 µg/ml;
Lab B: 0, 125, 250, 500, 750, 1000, 1500, 2000 µg/ml;
LAB C: 0, 50, 68, 100, 125, 200, 250, 400, 500, 800, 1000, 1600 µg/ml
Result : positive
Control group :
Method : other: see freetext ME
Year : 1988
GLP : no data
Test substance : other TS: o-Toluidine hydrochloride
Method : Assays for morphological transformation were performed in three different
laboratories (Lab A,B, and C), according Tu et al , 1986.
The basis protocol required that 15-20 20-mm tissue culture plates
containing 300 target and 6x10E4 feeder cells in 8 ml medium be used for
each treatment.
Lab. B used five different cell pools during these studies and for three of
these pools, higher levels of transformation were obtained when feeder and
target cell numbers were reduced to 2x10E4 and 200 or 150 cells,
respectively.
Labs A and B used different methods for chemical delivery. In Lab A,
chemicals were dissolved and serially diluted in the solvent (usually water
or DMSO). A 20µl volume containing the requisite chemical concentration
was then added to 8 ml medium in each plate. In Lab B, the chemicals
were dissolved in solvent initially but diluted subsequently in complete
medium. The chemical dose was then added in a 4 ml volume to plates
contain in 4 ml of medium.
Lab. C used both "direct" and "indirect" methods of chemical delivery. In
some assays, Lab.C refed the cells with fresh medium containing no
chemical on day 3. Each assay was terminated after 7 days and the plates
were rinsed, fixed with methanol, and stained with Giemsa. The number of
surviving colonies and the frequency of colonies with altered morphologies
were determined.
Remark : Summary report of mammalian cell transformation assays in three different
laboratories.
Result : o-Toluidine hydrochloride was consistently positive in all the assays
conducted by the three laboratories.
Reliability : (4) not assignable
special study: method evaluation, comparison of different labs
25.05.2004 (376)
Species : other: mouse; mammalian cell transformation assay
Sex :
Strain : other: Balb/c-3T3 mouse embryo cells
Route of admin. :
Exposure period :
Frequency of treatm. :
Post exposure period :
Doses :
Result : positive
Control group :
Method : other: see freetext TC
Year : 1985
GLP : no data
UNEP PUBLICATIONS 259