o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
5. TOXICITY ID: 95-53-4
DATE: 07.01.2005
132
hydrochloride:
1) The concentration of o-toluidine in blood was observed for a period of 6
hours by measuring according to Brodie and Axelrod (1948): J. Pharmacol.
exp. Ther. 94, 22
2) Products from N-oxidation were extracted from blood with carbon
tetrachloride and determined with the method of Herr and Kiese (1959),
Naunyn-Schmiedeberg's Arch.exp. Path. Pharm. 235, 351
3) Hemiglobin was estimated by measuring the increase of the extinction at
550 mµ that was caused by adding cyanide to a blood solution of pH=6.8
Result : Results were only reported as graphics:
1) Graphic: Concentration: µg o-toluidine per ml blood versus time course
in hours:
Half-life time of plasma elemination was: approximately half an hour. 7
hours post application approximately 10 µg o-toluidine per ml of blood was
found.
2) Graphic: Hemiglobin content per total blood pigment versus time course
in hours:
increased with increasing time from application, max value was reached 6
hours post application indicating the presence of a reactive oxidation
product.
3) Carbon tetrachloride extracts did not contain o-nitrosotoluene
Reliability : (2) valid with restrictions
Data of the results were only shown as graphics, no data on the purity of
the TS
Flag : Critical study for SIDS endpoint
02.06.2004 (132)
In Vitro/in vivo : In vivo
Type : Metabolism
Species : rat
Number of animals
Males :
Females :
Doses
Males : 0.82 mmol/kg b.w. in corn oil
Females :
Vehicle : other: corn oil
Route of administration : s.c.
Exposure time :
Product type guidance :
Decision on results on acute tox. tests :
Adverse effects on prolonged exposure :
Half-lives : 1 st :
2 nd :
3 rd :
Toxic behaviour :
Deg. product :
Method : other: Detection of the urinary metabolite N-hydroxy-o-toluidine with HPLC
Year : 1983
GLP : no data
Test substance : other TS: o-toluidine, purity h.p.l.c. grade
Result : The amounts of N-hxdroxy-o-toluidine found in the 0-6h urines of rats,
treated with 0.82 mmol/kg bw o-toluidine, ranged from 0.04 to 0.36 µmol.
Test condition : Male F344 rats (230-260 g b.w.) were s.c. injected with 0.82 mmol/kg bw o-
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