o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
3. ENVIRONMENTAL FATE AND PATHWAYS ID: 95-53-4
DATE: 07.01.2005
84
Type of measurement : concentration at contaminated site
Media : other: tobacco smoke and ambient air
Concentration :
Method : GC/MS
Method : - GC (Hewlett-Packard 5890 series II and MSD 5971A, Superox II column
from Bio-Rad)
- 13C1-o-toluidine was obtained from Cambridge Isotope Laboratory
- Smoking machine with continuous aspiration of 21 ml/s main stream and
138 ml/s side stream
- Absorption of cigarette smoke in acidic solution
- Washing on Florisil column
- Eluate directly injected into GC/MS
- Ambient air sampling via a pump: 33 ml/s (DuPont P4000 with
automatically adjusted flow), duration 8.33 h
- Sample preparation similar to that of cigarette smoke.
Result : Both the main stream smoke of cigarettes, (which is inhaled by the smoker)
and the side stream smoke of cigarettes (which is also inhaled by the non
smoker) contain significant amounts of all toluidine isomers and other
aromatic amines. Depending on the brand, the o-toluidine content is 30-
208 ng/cigarette in the main-stream smoke, and 10-100 times more in the
side-stream smoke (2-4 µg/cigarette).
In air, there are several aromatic amines (tracers of cigarette smoke).
There is a strong correlation of o-toluidine levels (ng/m3) in indoor air with
the smoking status of the inhabitants:
- Outdoors 2.5
- Apartment of a non smoker 5.1
- Office of a non smoker with smokers in contiguous room 6.3
- Office of a non smoker with smokers in contiguous room after overnight
ventilation 3.0
- Office with 1 smoker 5.4
- Office with 2 smokers 12.8
- Club room 16.9
- Non-smoking train compartment 6.5
- Hair-dresser saloon 10.4
Reliability : (2) valid with restrictions
Basic data given
Flag : Critical study for SIDS endpoint
07.06.2004 (65)
Type of measurement : concentration at contaminated site
Media : biota
Concentration :
Method : GC/MS
Method : - VOC of human breath was anaylsed by GC/MS
- 10 cancer patients with bronchogenic carcinoma
- 16 control subjects
- All persons were questioned on their food intake during last 24 h prior to
test, additionally medication history was compiled
Remark : Contamination of former smokers by environmental tobacco smoke should
have been taken into account.
Result : Lung cancer patients exhaled significantly greater amounts of o-toluidine
(median value: ca. 6 ng/20 l lung air) than age-matched controls (median
value: ca. 3 ng/20 l lung air). Since o-toluidine was detected in all patients
who have not smoked for 3-15 years, it was discussed that o-toluidine
might also stem from broiled food due to pyrolysis of amino acids and from
vegetables.
Reliability : (2) valid with restrictions
Basic data given
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