o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
5. TOXICITY ID: 95-53-4
DATE: 07.01.2005
256
(1) 0.24 %, 2.4 %, 1.2 %
(2) 0.0 %, 0.34 %, 0.68 %
(3) 0.26 %, 1.6 %, 0.0 %
(4) 0.25 %, 1.8 %, 0.67 %
The highest transformation frequency was obtained at 100 µg/ml, and the
frequency decreased at higher concentrations of the compound.
The positive control was functional.
Test condition : Chemicals were administered to the SHE cells in petri dishes.
After an incubating period of 6 days the cells were washed free of the
chemicals and the colonies were fixed and stained for counting and
examination.
Tests were performed without exogenous metabolic activation
Positive controls with benzo[a]pyrene
Negative controls with DMSO
4 trials were performed
To judge a substance positive in the assay, it was required that the test
substance should give transformation frequencies high than 1 % in at least
2 independent experiments.
Reliability : (2) valid with restrictions
no data on GLP and on purity of tthe testsubstance
25.05.2004 (370) (371)
Species : other: hamster; mammalian cell transformation assay
Sex :
Strain : other: Syrian hamster embryo cells
Route of admin. :
Exposure period : 24 hours
Frequency of treatm. :
Post exposure period :
Doses : 0, 300, 600, 900, 1200 µg/ml culture medium
Result : positive
Control group : yes
Method : other: standard Syrian hamster embryo cell transformation assay modified
for reduced pH in the culture medium
Year : 1996
GLP : no data
Test substance : other TS: o-Toluidine hydrochloride
Result : o-toluidine in a 24h exposure SHE cell transformation assay yielded
significant morphological transformations at four doses from 750 to 1200
µg/ml, while achieving 38% cytotoxicity at the top dose.
Higher dose of this chemical were not tested because of insolubility in the
solvent.
The trend test result for this dataset was p=0.0000, and therefore the
overall SHE cell transformation assay call for o-toluidine was positive.
The positive control was functional.
Test condition : A preliminary cytotoxicity assay
is conducted to establish an appropriate dose range for the test chemical:
The top dose of the test chemical should result in 50 % or greater
reductiomn in plating efficiency. The low dose of the assay will be the
highest concentration of the test chemical which results in 90-100 %
plating efficiency.
Standard SHE cell transformation assay
modified for reduced pH at 6.7 in the culture medium.
Chemicals were administered to the SHE cells in petri dishes.
After a 24h treatment period the cells were washed free of the chemicals
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