o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
3. ENVIRONMENTAL FATE AND PATHWAYS ID: 95-53-4
DATE: 07.01.2005
96
containing 40 ml of nutrient solution.
- Bottles were incubated in the dark at 25 °C.
- Contact time was up to 64 days including adaptation period.
- Ring cleavage was checked by decrease of absorbance at 265 nm,
measured after centrifugation in the supernantant. Precipitates and
supernatants were returned to the appropriate reaction bottles.
- Control tests were performed with identical samples except that 8 mg of
HgCl2 and 5E-07 M Tween 80 were added to each bottle.
- Tests for toxicity of the test substances to microorganisms were done on
identical samples however using glucose as an additional source of
carbon.
Reliability : (2) valid with restrictions
Basic data given
20.02.2004 (79)
Type : aerobic
Inoculum : other:
Concentration : 5.35 mg/l related to Test substance
related to
Contact time :
Degradation : 63.8 (±7.9) % after 96 hour(s)
Result :
Deg. product :
Method : other
Year : 2002
GLP : no
Test substance : other TS: o-Toluidine, Sigma (purity > 98%, determined by HPLC)
Method : An abiotic system consisting of Mn(III) in oxalate buffer under aerobic
conditions (Mn(II)/oxalate/O2) was used to transform 2-amino-4,6dinitrotoluene
and its derivatives (including o-toluidine).
Study elucidated the role of the manganese peroxidase (MnP) from whiterot
fungi.
Remark : Very special test conditions
Result : 63.8+/-7.9 % of the initial concentration of o-toluidine were transformed
presumably by superoxide radicals formed during the reaction of cleavage
of oxalate to -COO-radicals.
These radicals reacted with oxygen to yield superoxide radicals which in
water become protonated to effective –HOO radicals.
Test condition : 50 µM (5.35 mg/l) of the test substance were incubated for 96 hours at 20
°C in a system containing oxalate and Mn(III), under aerobic conditions.
The reaction mixture was sterilized. pH measured in the mixtures rose from
4.5 to 7.5-8.0 over the time of the experiment.
Quantitative determination of nitroaromatic compounds was performed by
reversed phase HPLC.
Reliability : (3) invalid
Unsuitable test system
29.01.2004 (46)
Type : aerobic
Inoculum : other: Water of river Soghua, China
Concentration : 2 mg/l related to COD (Chemical Oxygen Demand)
related to
Contact time :
Degradation : 41 (±) % after 5 day(s)
Result :
Deg. product :
Method : other: Determination of biodegradation rate constant
Year : 2001
GLP : no
Test substance : other TS: o-Toluidine, reagent grade
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