o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals
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OECD SIDS o-<strong>TOLUIDINE</strong><br />
5. TOXICITY ID: <strong>95</strong>-<strong>53</strong>-4<br />
DATE: 07.01.2005<br />
216<br />
Test concentration : 0, 0.001, 0.0001, 0.00001 M in DMSO<br />
Cycotoxic concentr. : 0.001 M in DMSO<br />
Metabolic activation : no data<br />
Result : negative<br />
Method : other: in vitro hepatocyte assay according Maslansky and Williams (1982),<br />
In Vitro 18, 683, incubation time: 4 h, autoradiographic measurement<br />
Year : 1987<br />
GLP : no data<br />
Test substance : other TS: o-toluidine, purity: 99 %<br />
Result : o-toluidine failed to induced a positive DNA repair response in either rat or<br />
hamster hepatocytes over the concentration range of 10E5 - 10E3-3<br />
Reliability : (4) not assignable<br />
no positive controls available<br />
15.07.2004 (294) (2<strong>95</strong>)<br />
Type : Unscheduled DNA synthesis<br />
System of testing : primary hepatocytes (rat)<br />
Test concentration : 8 doses with the range of 1000 - 0.5 nmole/ml<br />
Cycotoxic concentr. : no data<br />
Metabolic activation : no data<br />
Result : negative<br />
Method : other: preparation of hepatocytes: according to Williams et al. 1977, In Vitro<br />
13, 809-817; autoradiographic assay according to Probst et al. 1981,<br />
Environ. Mutagen 3, 11-32<br />
Year : 1983<br />
GLP : no data<br />
Test substance : other TS: o-toluidine, no data on purity<br />
Method : solvent: DMSO<br />
positive control MNNG. 2-AAF<br />
Reliability : (4) not assignable<br />
documentation insufficient for assessment<br />
15.07.2004 (213)<br />
Type : Unscheduled DNA synthesis<br />
System of testing : HeLa S3 cells<br />
Test concentration : 25 µg/ml (dose of maximal response)<br />
Cycotoxic concentr. : no data<br />
Metabolic activation : with and without<br />
Result : positive<br />
Method : other: scintillation counting of extracted DNA<br />
Year : 1985<br />
GLP : no data<br />
Test substance : other TS: o-toluidine, no data on purity<br />
Test condition : incubation time:<br />
2.5 hours<br />
METABOLIC ACTIVATION:<br />
S9 mix liver homogenates from Aroclor 1254 induced rats<br />
CONTROLS:<br />
positive - 3,3'-dichlorobenzidine with and 4-nitroquinoline-1-oxide without<br />
S9 mix<br />
negative - concurrent solvent controls (DMSO)<br />
Definition of positive results:<br />
Student's t-test for equal variance.<br />
Reliability : (4) not assignable<br />
test procedure evaluation, no data on cytotoxicity, no data on test<br />
concentration<br />
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