o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
4. ECOTOXICITY ID: 95-53-4
DATE: 07.01.2005
Test condition : - Adult amphipods were selected and acclimated at a temperature of 23 °C
for 7 days before experiments began.
- 7 test substance concentrations were tested (range: 0.15-40 mg/l)
- 20 adult amphipods were used for each concentration. Test amphipods
were placed in a culture bowl containing 200 ml of test solution and were
fed with mixtures of ground sea lettuce and fish flake. The bowls were
covered with glass plates. Test media were changed daily and experiments
lasted at least 7 days.
- Temperature= 23+/-1.7 °C; Salinity of seawater= 30 promille.
- Mortality was daily recorded.
Reliability : (2) valid with restrictions
Test procedure in accordance with generally accepted scientific standards
and described in sufficient detail
18.06.2004 (103)
Type : static
Species : other: Dugesia tigrina (plathelminthes)
Exposure period : 96 hour(s)
Unit : mg/l
EC50 : 143.9
Analytical monitoring : no data
Method : other
Year : 1999
GLP : no
Test substance : other TS: o-Toluidine, purity is not specified
Method : - The method was described in Piontek M (1999). Use of the planarian
Dugesia tigrina Girard in toxicological studies of acute intoxication. Pol.
Arch. Hydrobiol. 46, 41-48.
The method was developed to test the acute toxicity of substances in
aquatic environment to Dugesia tigrina. The criterion was the regeneration
progress of cut individuals after 10 days which leads either to the renewal
of the lacking body parts or to animal´s death.
- Dead animals were counted after 96 h in the test with the whole
planarians and after 240 h in the test with cut planarians.
- Physiological state of D. tigrina was tested with potassium dichromate
according to ISO Document (1980). Determination of acute lethal toxicity of
substances to freshwater fish using a semi-static procedure. ISO/TC
147/SC 5/GT 3 N 38.
Result : Other results:
with whole individuals
96 h-LC10 = 108.1 mg/l
with cut individuals
240 h-LC10 = 1.7 mg/l
240 h-LC50 = 5.0 mg/l
Test condition : - The planarians were cultured under the following conditions: temperature:
18-22 °C, pH = 7.2-8.2, Oxygen content above 5.0 mg O2/l. The planarians
were fed with Daphnia ad libitum and kept at daylight with natural day/night
rhythm.
- Experiments were carried out in 50 ml flasks.
- The standard water prepared according to the ISO procedure had a
hardness of 100 ppm expressed as calcium carbonate.
- In each flask 10 test organisms were placed (10 whole planarians in 96 h
experiments and 5 planarians each cut into 2 parts in 240 h tests). The
experiments were done in triplicate.
- The individual animals were 11-12 mm long and 20 days old.
Reliability : (2) valid with restrictions
Basic data given
16.07.2004 (104)
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