o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
5. TOXICITY ID: 95-53-4
DATE: 07.01.2005
cells were treated for 6 hours with or without 5% S9-mix
METABOLIC ACTIVATION:
S9 mix liver homogenates from phenobarbital and 5,6-benzoflavone
induced rats
Controls:
negative controls:
untreated cultures; vehicle (DMSO) treated cultures;
positive control:
Dimethylnitrosamine (DMN)
Reliability : (4) not assignable
no data on cytoxicity
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Type : Chromosomal aberration test
System of testing : RL4 cells
Test concentration : 0, 87.5, 175, 350, 700 µg/ml DMSO
Cycotoxic concentr. : 500 - 750 µg/ml DMSO
Metabolic activation : without
Result : positive
Method : other: chromosomal aberration test ( see freetext TC)
Year : 1985
GLP : no data
Test substance : other TS: o-toluidine, no data on purity
Result : Over the dose-range 87.5-700µg/ml o-toluidine induced a dose related
increase in chromatid gaps that was significant at 700 µg/ml.
In the absence of other chromatid or chromosome aberrations the increase
in gaps alone is regarded as indicative of weak clastogenic activity.
Test condition : ---Test system
RL4 cells (epithelial-like cell isolated from liver of 10-day-old male Wistar
rat, cycle time: 13 h)
---Preliminary cytotoxicity test
Cytotoxicity was measurement as plating efficiency
Cytotoxicity reduced by 50% between 500 and 750 µg/ml and 700 µg/ml
was used as the highest dose level.
---Incubation time:
22 hoursadditional 2 hrs after addition of colcemid solution
---Controls
positive controls: 7,12-dimethylbenzanthracene 1µg/ml
negative controls: solvent (DMSO)
Reliability : (4) not assignable
Method evaluation, unsuitable cell system
15.07.2004 (180) (281) (282)
Type : Chromosomal aberration test
System of testing : CH1-L (fibroblasts from the liver of Chinese hamster)
Test concentration : 0, 12, 30, 60, 120 µg/ml DMSO
Cycotoxic concentr. : preliminary test, tests carried out with dose up to 50% growth inhibition
Metabolic activation : without
Result : positive
Method : other: chromosome aberrations and aneuploidy
Year : 1985
GLP : no data
Test substance : other TS: o-toluidine, no data on purity
Result : All concentrations tested, both excluding and including gaps, caused
significant increase in the level of chromosome aberrations. Although doseresponse
was observed, this was not statistically significant.
A similar situation was found with regard to aneuploidy levels. The higher 2
doses had significant greater levels of hyperdiploidy, but dose-response
was non-significant.
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