o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
3. ENVIRONMENTAL FATE AND PATHWAYS ID: 95-53-4
DATE: 07.01.2005
Method : The test substance was added to 250 ml biochemical oxygen demand
(BOD) bottles. The bottles were then filled to capacity with the water
sample, sealed and incubated for 5 days at 20+/-1 °C.
- Two replicates were conducted for each chemical and each control
(inoculum only).
- The dissolved oxygen concentrations were determined by iodometric
titration method.
Remark : It is not clear in which study the measurements were done,
and which study cites the other.
Result : A biodegradation rate constant of 0.65 (1/d) was determined for o-toluidine.
Test condition : - Water samples were gathered from the Jilin Province section in the
Songhua river. No large industry enterprises or new pollutant sources are
present in the vicinity of this section.
- Temperature of the original water samples ranged between 15 and 20 °C,
the concentration of dissolved oxygen ranged between 7.8 and 9.0 mg/l,
and the pH was between 6.8 and 7.0.
- The bacteria counts were determined by standard plate count techniques
and were about 1200 to 3000 colony forming units /ml.
- Medium was composed of 3 g of beef extract, 10 g of peptone, 20 g of
agar and 1 l of distilled water. The pH of the culture medium was adjusted
to 7.6 and the culture was sterilized for 20 min. at 121 °C. 1 ml of diluted
water sample was cultivated in 15 ml of the above medium at 31 °C for 24
h.
- The added concentration of chemicals was approximately 2 mg/l on the
basis of their theoretical oxygen demand and residual dissolved oxygen of
at least 1 mg/l at the final day.
Reliability : (3) invalid
Documentation insufficient for assessment
15.06.2004 (80) (81) (27)
Type : aerobic
Inoculum : Aerobacter sp. (Bacteria)
Concentration : 500 mg/l related to Test substance
related to
Deg. product :
Method : other
Year :
GLP : no
Test substance : other TS: o-Toluidine, purity is not specified
Method : Soil and marine samples from various parts of the world were used in order
to be able to isolate and study the naturally occuring microorganisms.
The capability of the various microorganisms to degrade selected organic
molecules and the maximum toxicant concentration to which they would
adapt were determined.
Result : - Aerobacter sp. was identified to be responsible for the degradation of otoluidine.
- 100 % ring-cleavage was observed within 64 h of incubation.
- When extremely effective mutants, selected by irradiation and cultivated
in sewage sludge were used, 100 % ring cleavage was observed already
after 6 h incubation.
Test condition : - Ring-cleavage by Aerobacter species, isolated from seawater and soil
samples;
- Inoculum was adapted for 21 days to the test substance.
- Temperature=30 °C
- Warburg studies were carried out at the designated temperatures and
with a fluid volume of 5.0 ml. All flasks and manometers were calibrated
under these conditions. The completely assembled flasks and manometers
were allowed to equilibrate for 15 minutes before readings were taken.
- Duration of the tests = 5 days.
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