o-TOLUIDINE CAS N°: 95-53-4 - UNEP Chemicals

OECD SIDS o-TOLUIDINE
4. ECOTOXICITY ID: 95-53-4
DATE: 07.01.2005
Unit : mg/l
EC50 (pH=6.0) : 8.9
EC50 (pH=7.8) : 6.6
EC50 (pH=9.0) : 3.5
Analytical monitoring : no data
Method : other: not specified
Year : 2000
GLP : no data
Test substance : other TS: o-Toluidine, purity is not specified
Method : The study assessed the toxicity of 13 anilines and 11 phenols to Daphnia
magna at pH values of 6.0, 7.8 and 9.0.
Result : The results were considered valid if dissolved oxygen measured at the end
of the test was at least 60 % of saturation and if the percentage of
immobilization observed for the controls was zero. Based on control
experiments it is known that Daphnia can survive only between pH 6.0 and
9.0 in water, at pH values greater than 9.0 and less than 6.0, mortality was
observed.
Results are given as log (1/EC50), where EC50 is given in
mol/l.
pH=6.0 log(1/EC50) = 4.08 (EC50=8.9 mg/l)
pH=7.8 log(1/EC50) = 4.21 (EC50=6.6 mg/l)
pH=9.0 log(1/EC50) = 4.48 (EC50=3.5 mg/l)
Test condition : - Daphnia magna were cultured parthenogenetically in an environmental
chamber at a temperature of 22+/-2 °C, with a photoperiod of 14 h daylight
/ 10 h darkness.
- 6-24 h old Daphnids were used for the toxicity tests. They were fed with a
diet of green algae.
- Acute toxicity tests were conducted with 10 animals in 25 ml of test water.
- At each pH, 6 concentrations of the test substance were tried. 3
determinations were performed each time.
- 3 different pH were tested: 6.0, 7.8 and 9
Reliability : (2) valid with restrictions
Study meets generally accepted scientific principles
20.01.2004 (28)
Type : static
Species : Daphnia magna (Crustacea)
Exposure period : 24 hour(s)
Unit : mg/l
EC0 : 2.5
EC50 : 26
EC100 : 60
Analytical monitoring : no
Method : other: immobilization test according to Bringmann G and Kuehn R
Year : 1977
GLP : no
Test substance : other TS: o-Toluidine, purity is not specified
Remark : - Effect endpoint: immobilization
- Effect values refer to nominal TS concentrations
Test condition : - Test organism: clone of wild population of Daphnia magna
- Cultures of Daphnia magna were fed daily with green algae (Chorella
vulgaris)
- Triplicate parallel dilution series with 10 daphnids per vessel of 20 ml (age
max. 24 h)
- Temperature: 20-22 °C
- Water source: tap water free of chlorine saturated with oxygen; hardness
16 ° (degree dH (German water hardness)) corresponding to 286 mg/l
CaCO3, pH=7.6-7.7
Reliability : (2) valid with restrictions
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