m-Cresol - ipcs inchem

OECD SIDS
m- / p-CRESOL
m/p-Cresol
An Ames test was performed without S9-mix and with S9-mix from rat and hamster livers. The
studies gave no indication of gene mutations (NTP 1991).
Conclusion:
In vitro, m-,and p- cresol did not induce gene mutations in bacterial and mammalian cell systems
and m/p-Cresol mixture did not induce gene mutations in bacteria, both in the presence or absence
of metabolic activation.
(B) Cytogenicity
m-Cresol:
There is a study on cytogenicity (Chromosome aberration) using Chinese Hamster Ovary (CHO)
cells in vitro which corresponds to the current OECD guideline 473 (Hazleton Lab. Am. 1988b).
The study gave no indication of any clastogenic activity of the substance.
In addition, there is a mouse lymphoma assay (Hazleton Lab. Am. 1988a) with a negative result. In
a Sister Chromatid Exchange (SCE) test on human fibroblasts without metabolic activation no
increases in exchange rates were seen (Cheng and Kligerman 1984).
p-Cresol
There is a study on cytogenicity (Chromosome aberration) using Chinese Hamster Ovary (CHO)
cells in vitro which corresponds to the current OECD guideline 473 Incubated without metabolic
activation the assay was positive in all doses. The metabolic activated cultures which were
incubated for 10 hours yielded negative results and those which were incubated for 20 hours yielded
positive results (Hazleton Lab. Inc. 1988f). In addition, there is a mouse lymphoma assay with a
negative result both in the presence or absence of metabolic activation (Hazleton Lab. Inc. 1988e).
In a Sister Chromatid Exchange (SCE) tests with human lymphocytes using a treatment time of up
to 90 hours (Jansson et al. 1986) and with human fibroblasts incubated with p-cresol for two hours
(Cheng and Kligerman 1984), no increases in exchange rates were seen.
m/p-Cresol
There are no cytogenetic assays in vitro with a m/p cresol mixture.
Conclusion:
m-Cresol did not induce chromosomal aberrations in vitro, whereas p-cresol had clastogenic
activity in CHO cells in both the presence or absence of S-9 mix. It is therefore possible that m/pcresol
mixture has the potential to induce chromosomal aberrations in vitro. Neither m- nor p-cresol
did increase SCE in vitro.
(C) Indicator test
m-Cresol
No induction of Unscheduled DNA Synthesis (UDS) was found in rat primary hepatocytes m
(Hazleton Lab. Am. Inc. 1988c). In contrast, UDS was induced in SHE cells, but only in the
presence of a metabolic activation system (Hamaguchi and Tsutsui 2000).
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