m-Cresol - ipcs inchem

OECD SIDS
m- / p-CRESOL MIXTURE
5. TOXICITY ID: 15831-10-4
DATE: 24.05.2004
Year : 1991
GLP : yes
Test substance : other TS: m-/p-cresol (60%:40% mixture)
Method : SIZE OF STUDY GROUP:
5 male and 5 female rats per group
TIME HELD BEFORE STUDY: 13-15 days
METHOD OF ANIMAL DISTRIBUTION:
randomized for each sex on the basis of body weight into
groups per sex
DIET: NIH-07 rat ration
ANIMAL ROOM ENVIRONMENT:
temperature: 72° +/-3° F, humidity: 50 % +/-15 %,
Fluorescent light: 12 hrs/day, room air changes : 10-12
changes/hr
TYPE AND FREQUENCY OF OBSERVATION:
observed twice daily, body weight taken initially, weekly,
and at termination, feed consumption by cage recorded twice
weekly
NECROPSY AND HISTOLOGIC EXAMINATION:
necropsy and tissue collection performed for all animals. A
complete histopathologic examination was conducted on all
control animals, all animals in the highest dose group with
at least 60 % survivors at study termination, and all
aninmals in higher dose groups inclusive of early deaths.
The following organs and/or tissues were included in
complete histopathological examinations, as well as any
tissue masses, gross lesions, and associated regional lymph
nodes: adrenals, aorta, bone (sternebrae, femur, or
vertebrae, including marrow), brain, bronchi, clitoral
gland, epididymis, oesophagus, heart, kidney, large
intestines (caecum, colon, rectum), liver, lungs, lymph
nodes (mesenteric), mammary glands, nasal cavity and
turbinates, oral cavity, ovaries, pancreas, parathyroids,
pharynx, pituirary, preputial gland, prostate, salivary
glands, scrotal sac, seminal vesicles, skin, small intestine
(duodenum, ileum, jejunum), spleen, stomach, testes, thymus,
thyroid, tongue, trachea, tunica vaginalis, urinary bladder,
uterus and Zymbal's glands.Target organs and gross lesions
were examined at lowere doses until a no-observed chemical
effect was determined. Target organs included the following:
nasal epithelium, bone marrow, forestomach, oesophagus,
thyroid and uterus. Organ weights recorded for brain, liver,
right kidney, thymus, heart, and lungs of all animals, and
the right testis of all males.
STATISTICAL METHODS:
nonparametric multiple comparison test of Dunn and Shirley,
Jonckheere's test
Remark : mean compound consumption (mg/kg bw/day):
males females
0 ppm 0 0
300 ppm 26 27
1000 ppm 90 95
3000 ppm 261 268
10000 ppm 877 886
30000 ppm 2600 2570
Result : All rats survived.
30000 ppm: Body weight gain was significantly reduced in
males and females, feed consumption was depressed during
the first week, all rats had thin appearance by day 6 not
beyond d 7.
UNEP PUBLICATIONS 359