m-Cresol - ipcs inchem

OECD SIDS
m- / p-CRESOL MIXTURE
5. TOXICITY ID: 15831-10-4
DATE: 24.05.2004
(contr.); exposure period, F0: 7d prior to cohousing, 98 d
(14 w) of continuous breeding, then pairs were seperated and
any litters born after the final litter were reared by the
dams until weaning, at the end of week 15: evaluation of
clinical signs, parenteral bw, fertility (number producing a
litter/number of breeding pairs), litters per pair, live
pups per litter, proportion of pups born alive, sex of live
pups, pup body weights within 24 hrs of birth, feed and
water consumption.
Task 3:
determination of the affected sex only when pos. effects on
reproductive function during task 2:
one week crossover mating trial with parental mice from
control and high dose groups, vaginal cytology evaluation
for 12 d prior to sacrifice; low dose and mid dose mice were
hold for necropsy together with task 3 mice: body and organ
weight determination and sperm evaluation of all males.
Task 4:
offspring assessment: mice born after week 15 were kept
until maturity and cohabited for 7 d and housed singly until
delivery, collection of vaginal smears, body and selcted
organ weights, epididymal and testicular spermatozoa
evaluation, mating index, pregnancy index, fertility index,
live F2 pups per litter, proportion of F2 pups born alive,
sex ratio, live F2 pup weight, adjusted live F2 pup weight,
average dam weight, average number of days to litter
Statistical methods:
Test for linear trend: ANOVA
Turkey's test for pairwise compairison to controls
nonparametric multiple comparison procedures of Dunn and
Shirley as modified by Williams
Jonckheere's test
Cochran-Annitage test
Chi-square test
parametric analysis of covariance
F-test,
Dunn's test
Result : F0:
mortality:
7 mice died: 2 in the controlgr., 2 in the 1.5 % dose-gr., 1
in the 1.0 %-dose-gr., 2 in the 0.25 %-dose-gr.
1.0 and 1.5 %-group:
reduced body weight gain and feed consumption after 16 w,
especially in delivering and lactating dams
1.5 %-group:
decreased bw, increased liver and kidney weight
all mice:
reproductive competence was not affected, including inital
fertility, the proportion of pups born alive or the sex of
the pups born alive,
1.5 % -group:
adjusted live pup weight and the number of pups per litter
(both sexes) were decreased by 5% and 20%, respectively;
cumulative days to fifth litter were increased by almost 3 d
compared to control
1.5 %-group, males:
decreased epididymal and seminal vesicle weights by 10 and
21 %, respectively, but no change in testis weight, sperm
parameters or testicular and epididymal histology
Cross over mating did not clearly reveal the affected sex,
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