m-Cresol - ipcs inchem

OECD SIDS
p-CRESOL
5. TOXICITY ID: 106-44-5
DATE: 24.05.2004
Test concentration : treatment time: 20 hrs:
-S9-mix, 100, 150, 200, 301 ug/ml performed twice; +S9-mix: 301, 601,
902 ug/ml;
treatment time: 10 hrs:
+S9-mix: 150, 225, 300 ug/ml performed twice
Cycotoxic concentr. : Preliminary range-finding assays were performed (3.01-3010 µg/ml) to
determine cytotoxicity: -S9-mix: >=301 µg/ml; +S9-mix: >=100µg/ml
Metabolic activation : with and without
Result : positive
Method : OECD Guide-line 473
Year : 1987
GLP : yes
Test substance : other TS: p-cresol, 99.8% pure
Method : Duplicate CHO cultures were incubated for 20 hrs with 100-301 ug/ml of
the test substance in the nonactivation aberrations assay.
The metabolic activation cultures were treated with 100-300
ug/ml of the test substance in a 10 hour assay and with
301-902 ug/ml in a 20 hour assay.
Solvent: DMSO
positive control: Mitomycin C, cyclophosphamide
statistical evaluation: Fisher's Exact Test with an adjustment for multiple
comparisons
Result : nonactivation assay and incubation for 20 hrs:
Increases in chromosomally aberrant cells ranging between 6.5 % and 11
% cells with aberrations (versus 1.0% of solvent control) or between 4%
and 14 %.cells with aberrations (versus 2.0 % of solvent control),
respectively.
Positive control was functional in each trial
Incubation for 20 hours with metabolic activation:
Increases in the chromosomally aberrant cells ranging between 18 % and
40.5 % cells with aberrations(902 µg/ml was toxic, versus 1.5% of solvent
control) and between 17 % and 43 % cells with aberrations (902 µg/ml was
toxic, versus 3.0 % of solvent control), respectivly.
Posivive control was functional in each trial .
Incubation for 10 hours in the presence of S9-mix:no significant difference
to the solvent controls; positive controls were functional
Reliability : (1) valid without restriction
Flag : Critical study for SIDS endpoint
06.02.2004 (154)
Type : Mouse lymphoma assay
System of testing : L5178Y TK+/- mouse lymphoma cells
Test concentration : with activation: 0.256 ug/ml, 0.511 ug/ml, 0.767 ug/ml, 1.02 ug/ml, 1.53
ug/ml, and 3.07 ug/ml. without activation: 51.1 ug/ml, 102 ug/ml, 153
ug/ml, 204 ug/ml, 307 ug/l, and 409 ug/ml.
Cycotoxic concentr. : with activation: 7.98 ug/ml. without activation: 511 ug/ml.
Metabolic activation : with and without
Result : negative
Method : other: similar to OECD Guide-line 476, No differentiation between large
and small colony mutants see also freetext ME
Year : 1988
GLP : yes
Test substance : other TS: p-cresol, 99.8% pure
Method : S9-MIX: of rat liver was used as metabolic activation system
SOLVENT: DMSO,
POSITIVE CONTROLS: ethylmethane sulfonate, 3-methylcholantrene,
POSITIVE RESPONSE was indicated by a >= two-fold increase over the
concurrent background frequency
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