m-Cresol - ipcs inchem

OECD SIDS
p-CRESOL
5. TOXICITY ID: 106-44-5
DATE: 24.05.2004
Study description suffers from deficiencies: no information about
cytotoxicity and whether a metabolic activation system was used or not,
only summary results given
Flag : Critical study for SIDS endpoint
06.02.2004 (157)
Type : Ames test
System of testing : Salmonella typhimurium strains TA98, TA100, TA1535, TA1537, TA1538
Test concentration : 0, 0.5, 5, 50, 500, 5000 ug/plate dissolved in DMSO, highest dose cytotoxic
Cycotoxic concentr. : 5000 ug/plate
Metabolic activation : with and without
Result : negative
Method : other: see freetext ME
Year : 1975
GLP : no data
Test substance : other TS: p-cresol, purity : 98 %
Method : plate incorporation. method according to Ames, Mutat. Res. 31, 347
(1975), solv.: DMSO, S9-MIX: of Aroclor-pretreated rat liver as metabolic
activation. system
CONTROLS: as positive control:sodium azide,2-nitrofluorene, 9-
aminoacridine,2-aminoanthracene,
as solvent control: DMSO
DATA EVALUATION: Significance level for positive dose-response effects
were obtained with the Joncheere test
STATISTICAL ANALYSIS: Joncheere test
Remark : Positive controls were functional
Reliability : (1) valid without restriction
Flag : Critical study for SIDS endpoint
06.02.2004 (158)
Type : Sister chromatid exchange assay
System of testing : cultured male human fibroblasts
Test concentration : 0, 0.008, 0.8, 4, 8 mM diluted in 95 % EtOH, 10, 30 mM diluted in MEM
Cycotoxic concentr. : from 10 mM onwards
Metabolic activation : without
Result : negative
Method : other: see freetext ME
Year : 1984
GLP : no data
Test substance : other TS: p-cresol, > 99% pure
Method : p-Cresol was added to the cells and incubated, in
triplicate, at 37 C for 2 hours. Following exposure, the cells were washed,
reincubated in the absence of the test chemical for 48 hours, harvested
and SCE frequency and cell-cycle kinetics analysed.
SOLVENT: p-Cresol was dissolved in
95% ethanol at concentrations up to and including 8 mM and
in Eagle's minimum essential medium (MEM) at concentrations above this.
CONTROLS: 95% Ethanol and mitomycin C were used as negative and
positive controls respectively.
EVALUATION CRITERIA: positive if a dose-dependant significant incrase
in SCE frequencies compared to control is observed
STATISTICAL ANALYSIS: Dunnett's test
Remark : p-Cresol did not induce significant increases over the control SCE
frequencies. The positive control was functional.
p-Cresol caused a small but statistically significant
decrease in cell-cycle progression at 8 mM (864 mg/l) and
above, indicative of a small cytotoxic response.
Reliability : (2) valid with restrictions
only tested in the absence of metabolic activation and no information on
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