11.06.2013 Views

Field Guide of Discovery-based Exercises for - Aseanipm ...

Field Guide of Discovery-based Exercises for - Aseanipm ...

Field Guide of Discovery-based Exercises for - Aseanipm ...

SHOW MORE
SHOW LESS

Create successful ePaper yourself

Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.

Section 4 • Integrated Insect and Rodent Pests Management<br />

5 Collect GMF- and/or WMF-infected and healthy lepidopterous pest larvae in learning and<br />

adjoining fields <strong>of</strong> organically-grown vegetables;<br />

5 Place GMF- and/or WMF-infected and healthy lepidopterous pest larvae in a bottle with<br />

cap; Add enough water and shake vigorously until water has whitish tint;<br />

5 Pour suspension into a hand sprayer using a sieve to separate solids;<br />

5 Set up Petri dishes with healthy lepidopterous pest larvae. Spray suspension on healthy<br />

lepidopterous pest larvae in Petri dishes to multiply GMF and WMF;<br />

5 Incubate <strong>for</strong> 1-2 days under room temperature. Observe <strong>for</strong> fungal growth. If exposed<br />

lepidopterous pest larvae are totally colonized by fungus, repeat 3 rd and 4 th steps <strong>of</strong><br />

procedure;<br />

5 If enough GMF and WMF are produced, try spraying suspension on lepidopterous pest<br />

infested learning or adjoining fields;<br />

5 Observe daily <strong>for</strong> at least one week. Present observation and conduct participatory discussion.<br />

5. Synthesize and summarize output <strong>of</strong> small groups into one big group output. Draw up<br />

conclusions and recommendation from this exercise.<br />

some suggested questions <strong>for</strong> processing discussion<br />

❏ Did you observe different organically-grown vegetable crops infested with lepidopterous pests<br />

in learning and adjoining fields?<br />

❏ What control methods were employed by farmers against lepidopterous pests infesting their<br />

organically-grown vegetable crops?<br />

❏ What signs and symptoms were exhibited by lepidopterous pest larvae infected with GMF and<br />

WMF? Characterize.<br />

❏ How long did it take GMF and/or WMF to kill host lepidopterous pest larvae? Which instars<br />

was mostly affected by GMF and/or WMF?<br />

❏ What was the effect <strong>of</strong> GMF and/or WMF on non-lepidopterous pests?<br />

❏ Did you observe farmers producing and using biological control agents to manage lepidopterous<br />

pests in their vegetable fields?<br />

❏ Did you observe farmers using GMF and/or WMF as biological control agents against<br />

lepidopterous pests in their vegetable fields?<br />

❏ Is the use <strong>of</strong> green (Metarhizium anisopliae) or GMF and white (Beauveria bassiana) or<br />

WMF muscardine fungi as biological control agents effective against lepidopterous pests <strong>of</strong><br />

organically-grown vegetables?<br />

❏ How, how much, and when is the best time to apply GMF and WMF as biological control agents<br />

against lepidopterous pests <strong>of</strong> organically-grown vegetables?<br />

235

Hooray! Your file is uploaded and ready to be published.

Saved successfully!

Ooh no, something went wrong!