Sorted By Test Name - Mayo Medical Laboratories

LEGRP
89564
LEIS
86219
elevated as long as the IgG response remains elevated. Cross-reactions have been described with several
species of bacteria and mycoplasma.
Test Performed by: Focus Diagnostics, Inc.
5785 Corporate Avenue
Cypress, CA 90630-4750
Legionella species, Molecular Detection, PCR
Clinical Information: Legionnaires' disease was first recognized during a pneumonia outbreak at the
Legionnaires convention in Philadelphia in 1976. Investigators with the CDC isolated a novel,
gram-negative bacillus, later named Legionella pneumophila. It is now widely recognized that Legionella
pneumophila (and other members of the genus Legionella) cause Legionnaires' disease.
Useful For: Sensitive and rapid diagnosis of pneumonia caused by Legionella species
Interpretation: A positive PCR result for the presence of a specific sequence found within the
Legionella 5S rRNA gene indicates the presence of a Legionella species DNA, which may be due to
Legionella infection or environmental/water Legionella DNA in the specimen. A negative PCR result
indicates the absence of detectable Legionella DNA in the specimen, but does not rule-out legionellosis as
false-negative results may occur due to inhibition of PCR, sequence variability underlying the primers
and/or probes, or the presence of Legionella species in quantities less than the limit of detection of the
assay.
Reference Values:
Not applicable
Clinical References: 1. Cunningham SA, Sloan LM, Uhl JA, et al: Validation of a real-time PCR
assay for the detection of Legionella species in respiratory samples. Abstracts of the Annual Meeting of
the Association for Molecular Pathology, 2009 General Meeting, Kissimmee, FL, Nov. 19-22, 2009 2.
Hayden RT, Uhl JR, Qian X, et al: Direct detection of Legionella species from bronchoalveolar lavage
and open lung biopsy specimens: comparison of LightCycler PCR, in situ hybridization, direct
fluorescence antigen detection, and culture. J Clin Microbiol 2001;39(7):2618-2626 3. Diederen BM,
Kluytmans JA, Vandenbroucke-Grauls CM, Peeters MF: Utility of real-time PCR for diagnosis of
Legionnaires' disease in routine clinical practice. J Clin Microbiol 2008;46(2):671-677 4. MacDonell MT,
Colwell RR: The nucleotide sequence of the 5S rRNA from Legionella pneumophila. Nucleic Acids Res
1987;15(3):1335
Leishmaniasis (Visceral) Antibody, Serum
Clinical Information: Visceral leishmaniasis (kala azar) is a disseminated intracellular protozoal
infection that targets primarily the reticuloendothelial system (liver, spleen, bone marrow) and is caused
by Leishmania donovani, Leishmania chagasi, or Leishmania infantum (Leishmania donovani complex).
Transmission is by the bite of sandflies. Clinical symptoms include fever, weight loss, and splenomegaly;
pancytopenia and hypergammaglobulinemia are often present. Most (90%) new cases each year arise in
rural areas of India, Nepal, Bangladesh, Sudan, and Brazil but the disease has a worldwide distribution,
including the Middle East. Definitive diagnosis has required the microscopic documentation of
characteristic intracellular amastigotes in stained smears from culture of aspirates of tissue (spleen, lymph
node) or bone marrow. The detection of serum antibodies to the recombinant K39 antigen of Leishmania
donovani is an alternative non invasive sensitive (95%-100%) method for the diagnosis of active, visceral
leishmaniasis.
Useful For: Diagnosis of active visceral leishmaniasis
Interpretation: A positive result is consistent with a diagnosis of active visceral leishmaniasis.
Reference Values:
Negative
Current as of January 4, 2013 7:15 pm CST 800-533-1710 or 507-266-5700 or MayoMedicalLaboratories.com Page 1100