Sorted By Test Name - Mayo Medical Laboratories

MYCPN
85107
provides same-day results. The described PCR assay has replaced conventional culture for Mycoplasma
hominis at Mayo Medical Laboratories.
Useful For: Rapid, sensitive, and specific identification of Mycoplasma hominis from synovial fluid,
genitourinary, reproductive, and lower respiratory sources
Interpretation: A positive PCR result for the presence of a specific sequence found within the
Mycoplasma hominis tuf gene indicates the presence of Mycoplasma hominis DNA in the specimen. A
negative PCR result indicates the absence of detectable Mycoplasma hominis DNA in the specimen, but
does not rule-out infection as false-negative results may occur due to the following; inhibition of PCR,
sequence variability underlying the primers and/or probes, or the presence of Mycoplasma hominis in
quantities less than the limit of detection of the assay.
Reference Values:
Not applicable
Clinical References: 1. Waites KB, Taylor-Robinson D: Mycoplasma and Ureaplasma. In Manual
of Clinical Microbiology. 10th edition. Edited by J Versalovic. ASM Press, Washington, DC, 2011 2.
Stellrecht KA, Woron AM, Mishrik NG, Venezia RA: Comparison of multiplex PCR assay with culture
detection of genital mycoplasmas. J Clin Microbiol 2003;42:1528-1533 3. Kenny GE: Genital
mycoplasmas: Mycoplasma genitalium, Mycoplasma hominis, and Ureaplasma species. In Mandell,
Douglas, and Bennett's Principles and Practice of Infectious Diseases. Edited by GL Mandell, et al.
Churchill Livingstone, New York, 2008 4. Yoshida T, Maeda SI, Deguchi T, et al: Rapid detection of
Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum, and Ureaplasma urealyticum
organisms in genitourinary samples by PCR-microtiter plate hybridization assay. J Clin Microbiol
2003;41:1850-1855 5. Cunningham SA, Shannon SK, Rosenblatt J, Patel R: Comparison of culture and
rapid polymerase chain reaction detection of Mycoplasma hominis, Ureaplasma urealyticum and
Ureaplasma parvum from genitourinary specimens. 48th Annual Infectious Diseases Society of America
Meeting, 2010.
Mycoplasma pneumoniae Antibodies, IgG and IgM, Serum
Clinical Information: Mycoplasma pneumoniae is an important respiratory tract pathogen. Several
syndromes have been associated with the infection including pharyngitis, tracheobronchitis, pneumonia,
and inflammation of the tympanic membrane presenting as bullous myringitis. Mycoplasma pneumoniae
accounts for approximately 20% of all cases of pneumonia. Classically, it causes a disease that has been
described as primary atypical pneumonia. The disease is of insidious onset with fever, headache, and
malaise for 2 to 4 days before the onset of respiratory symptoms. Most cases do not require
hospitalization. Symptomatic infections attributable to this organism most commonly occur in children
and young adults (ages 2-19 years).(1)
Useful For: An aid in the diagnosis of disease associated with Mycoplasma pneumoniae
Interpretation: Positive IgM results are consistent with acute infection, although false positives do
occur (see Cautions). A single positive IgG result only indicates previous immunologic exposure.
Negative results do not rule-out the presence of Mycoplasma pneumoniae-associated disease. The
specimen may have been drawn before the appearance of detectable antibodies. If testing is performed too
early following primary infection, IgG and/or IgM may not be detectable. If a Mycoplasma infection is
clinically indicated, a second specimen should be submitted at least 14 days later.
Reference Values:
IgG
< or =0.90 (negative)
0.91-1.09 (equivocal)
> or =1.10 (positive)
IgM
< or =0.90 (negative)
0.91-1.09 (equivocal)
Current as of January 4, 2013 7:15 pm CST 800-533-1710 or 507-266-5700 or MayoMedicalLaboratories.com Page 1269