Sorted By Test Name - Mayo Medical Laboratories

BARTB
89983
BASL
82489
B. bacilliformis: historical pathogens of emerging significance. Microbes Infect 2000
August;2(10):1193-1205 2. Agan BK, Dolan MJ: Laboratory diagnosis of Bartonella infections. Clin Lab
Med 2002 December;22(4):937-962 3. Maguina C, Gotuzzo E: Bartonellosis. New and old. Infect Dis
Clin North Am 2000 March;14(1):1-22 4. Vikram HR, Bacani AK, Devaleria PA, et al: Bivalvular
Bartonella henselae prosthetic valve endocarditis. J Clin Microbiol 2007 December;45(12):4081-4084 5.
Lin EY, Tsigrelis C, Baddour LM, et al: Candidatus Bartonella mayotimonensis and endocarditis. Emerg
Infect Dis 2010 Mar;16(3):500-503
Bartonella, Molecular Detection, PCR, Blood
Clinical Information: Bartonella henselae and Bartonella quintana are small, pleomorphic,
gram-negative bacilli that are difficult to isolate by culture due to their fastidious growth requirements.
Bartonella henselae has been associated with cat scratch disease, bacillary angiomatosis, peliosis hepatitis,
and endocarditis. Bartonella quintana has been associated with trench fever, bacillary angiomatosis, and
endocarditis. The diagnosis of Bartonella infection has traditionally been made by Warthin-Starry staining
of infected tissue and and/or serology. However, these methods may be nonspecific or falsely negative,
especially in the early stages of disease. Evaluation of infected tissue or blood using PCR has been shown
to be an effective tool for diagnosing Bartonella infection. Mayo Medical Laboratories has developed a
real-time PCR test that permits rapid identification of Bartonella species. The assay targets a unique
sequence of the citrate synthase (gltA) gene present in Bartonella species.
Useful For: Diagnosing Bartonella infection where Bartonella DNA would be expected to be present in
blood, especially endocarditis. This test does not differentiate between Bartonella henselae and Bartonella
quintana.
Interpretation: A positive test indicates the presence of Bartonella species DNA. A negative test
indicates the absence of detectable DNA, but does not negate the presence of the organism or recent
disease as false negative results may occur due to inhibition of PCR, sequence variability underlying
primers and/or probes, or the presence of Bartonella DNA in quantities less than the limit of detection of
the assay.
Reference Values:
Not applicable
Clinical References: 1. Karem KL, Paddock CD, Regnery RL: Bartonella henselae, B. quintana, and
B. bacilliformis: historical pathogens of emerging significance. Microbes Infect 2000
August;2(10):1193-1205 2. Agan BK, Dolan MJ: Laboratory diagnosis of bartonella infections. Clin Lab
Med 2002 December;22(4):937-962 3. Maguina C, Gotuzzo E: Bartonellosis. New and old. Infect Dis
Clin North Am 2000 March;14(1):1-22 4. Vikram HR, Bacani AK, Devaleria PA, et al: Bivalvular
Bartonella henselae prosthetic valve endocarditis. J Clin Microbiol 2007 December;45(12):4081-4084
Basil, IgE
Clinical Information: Clinical manifestations of immediate hypersensitivity (allergic) diseases are
caused by the release of proinflammatory mediators (histamine, leukotrienes, and prostaglandins) from
immunoglobulin E (IgE)-sensitized effector cells (mast cells and basophils) when cell-bound IgE
antibodies interact with allergen. In vitro serum testing for IgE antibodies provides an indication of the
immune response to allergen(s) that may be associated with allergic disease. The allergens chosen for
testing often depend upon the age of the patient, history of allergen exposure, season of the year, and
clinical manifestations. In individuals predisposed to develop allergic disease(s), the sequence of
sensitization and clinical manifestations proceed as follows: eczema and respiratory disease (rhinitis and
bronchospasm) in infants and children less than 5 years due to food sensitivity (milk, egg, soy, and wheat
proteins) followed by respiratory disease (rhinitis and asthma) in older children and adults due to
sensitivity to inhalant allergens (dust mite, mold, and pollen inhalants).
Useful For: Testing for IgE antibodies may be useful to establish the diagnosis of an allergic disease
and to define the allergens responsible for eliciting signs and symptoms. Testing also may be useful to
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